Dear Fred,

Sadly, titebond is not available in South Africa. I have made extensive 
enquiries, but with no luck. So unless the formula is available (nothing on 
the Franklin website) I can't even guess for a substitute.

Thanks anyway



Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"


Dear Fred,

sa


----

Hi Louise.  I use a product called titebond.  It is a water
resistant wood glue.  If you are unable to find that brand I'm sure
that other wood glues would work.  I make an aqueous solution of 1%-5%
(it is thick and difficult to measure an exact amount) and add a grain
of thymol for preservative.  I spread a small drop on a slide and
allow it to dry before mounting the section.  It does a great job of
adhereing dense bone.  Unlike Elmers glue or gelatin, there is no
background staining.

Fred Underwood
Montgomery County Coroner
Dayton, Ohio
Subject: Re: coated slides Elmer's



	Dear all

	I have heard a lot about Elmer's Glue, which I gather is some
type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones,  used specifically for woodwork or crafts are
available.
So...my question is this: How is it used?  Neat, Diluted, in the
waterbath???  PLease advise as I have some deadful whole paw sections
that I
am having difficuly "sticking" to the slide.

	BTW, I have tried chrome alum, but get horrible background so my
thanks go
to Gayle for suggesting placing them in NBF. I will try that too.

	Best regards



	Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





	    At 08:48 a.m. 01/08/2003  0400, you wrote:
 >Hi,
 >I am having problems with tissue staying on my slides after drying
 >overnight
 >in 37C oven and then one more night in a 60C oven.  The tissue is
chicken
 >leg knee joints.   They are formalin fixed, EDTA decaled, and
paraffin
 >embedded samples.  I think that coated slides may help.  Does
anyone have a
 >simple protocol for making coated slides to help this tissue stick?
 >Thanks in advance for the help.
 >
 >Loralee Gehan
 >University of Rochester

	>   Loralle:
You  can  use  a  vinyl glue  like  Elmer#180#s, it
works  fine  in  cases
like you  are  describiyng. The  adhesion  properties  are
at  least
the  same  that  charged slides  when  you are  treating
samples
like  cartilage  and bone.
Good luck,Carlos.

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