What is the species of your tissue culture. If it is a human line then I
would think the human brain would be one good positive control. When working
with cell cultures I also like to use another cell line a s a negative
control. GFAP and a number of primaries can be tweaked in terms of
sensitivity so this may also be a consideration. I work with rodents and use
a normal brain as well as a brain inoculated with an agent that will cause
gliosis. I also use lung from the same species for a negative control. Any
way just some thoughts.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: david moses [mailto:kutaisi13@hotmail.com]
Sent: Friday, August 01, 2003 4:45 AM
To: histonet@pathology.swmed.edu
Subject: Controls for TC


Dear Histonetters

I'd appreciate your opinion on suitability of adult brain sections as 
controls(positive/negative) for immunocytochemistry performed in tissue 
culture on neurons and glia(such as tubulin,GFAP,TH etc').

David Moses
Research fellow
Monash Uni

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