RE: bkg IHC
From: | "Favara, Cynthia (NIH/NIAID)" |
Did I miss something?
What primary?
What controls?
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
-----Original Message-----
From: becky riesen [mailto:beckyr@naples.net]
Sent: Thursday, July 31, 2003 8:45 AM
To: histonet@pathology.swmed.edu
Subject: bkg IHC
I have specific staining in polys on my negative controls when the
section is a bone core from bone marrow biopsies. All other specimen
types seem to be fine. I have doubled the time in my H2O2 to 12
minutes. I have purchased new H2O2 in case I had an old bottle.
I'm increasing the time more, but I use an automated stainer and
this will lengthen the time of my entire run and I'm concerned about
getting too long here. I wonder if there are other suggestions,
solutions, procedures that you folks could share that may help my
situation. THANKS!
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