RE: Paneth cells

From:"Smith, Allen"

Although eosin works poorly, phloxine works well on human Paneth cells.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff@UMDNJ.EDU]
Sent: Friday, August 09, 2002 1:34 PM
To: Martin, Ronald
Cc: ''
Subject: Re: Paneth cells

    PAS works well on rat and mouse. An Alcian Blue-PAS sequence often
shows a pink granule surrounded by a blue rim in the mouse. Eosin does
not work too well on mouse Paneth cells, if my memory serves me
correctly. Biebrich Scarlet at an alkaline pH, as John Kiernan mentioned,
is also worth a try if you have some around in the lab. Older editions
(2nd, 3rd) of Humason mentioned a "diazotized safranin" method to stain
both Paneth cells and enterochromaffin cells but I never tried it.

"Martin, Ronald" wrote:

> Does anyone know of a special stain for paneth cells of the GI tract?
> Thanks,
> Ron

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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