Hi .  I have never tried it but our experience has been that AR specimens stain better with something like the
Vector Elite kit,which is a 2 step method so it takes a little longer. One hour in primary adn 30 minutes in
secondary and then 30 minutes in tertiary.
If that fails try  something like Dako's signal amplification kit CSA kit. Hope this helps
Violetta,
Pathology ,LHSC
London Ontario

Greg Dobbin wrote:

> Nafiesa,
> I am still struggling with my COX-2 staining as well; but you might try
> incubating your primary antibody overnight at 4 C.  Good luck.
>
> Date sent:              Mon, 19 Aug 2002 08:49:27 +0200
> From:                   "Allie, N, Nafiesa," 
> Subject:                Cox-2 antibody
> To:                     histonet@pathology.swmed.edu
>
> > Hi Everyone
> > I'm hoping that somebody will have some advise about the Cox-2
> > antibody from BD Transduction Laboratories, Cat#  610203.
> > I've tried antigen retrieval with Citrate Buffer, diluting the
> > antibody at 1:100, incubating for 30min, and using Envision(Dako),
> > with no success. If anyone can give some advise, it will be very
> > appreciated.
> > Thank you..
> > Nafiesa
> > #004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004#
> > Nafiesa Allie
> > Dept. Anat.Path.
> > Medical School
> > Univ.of Cape Town
> > Anzio Rd.
> > Observatory
> > 7925
> > Rep. of South Africa
> > #004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004#
> >
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> ~~~~~~~~~~~~~~~~~~~~~~