edge effects

From:
JHoffpa464@aol.com

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OK a question for my own gratification. What causes the so called edge effect 
i.e. increased none specific staining around the edge of sections, with IHC. 
I have never seen the effect with IF. I know what everyone says, it's from 
partial drying around the edge of sections. However I have seen it even in 
sections completely flooded.
John

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>OK a question for my own gratification. What causes the so called edge effect 
<BR>i.e. increased none specific staining around the edge of sections, with IHC. 
<BR>I have never seen the effect with IF. I know what everyone says, it's from 
<BR>partial drying around the edge of sections. However I have seen it even in 
<BR>sections completely flooded.
<BR>John</FONT></HTML>
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