RE: edge effects
|From:||"Morken, Tim" <email@example.com>|
I think the "edge effect" is cause by drying/modifiying of the tissue
surface during sampling and processing than during staining. The staining
merely highlights it. Maybe it has to do with the relatively extreme
exposure the of outside surface of the tissue sample. The interior is much
less affected, I think.
From: JHoffpa464@aol.com [mailto:JHoffpa464@aol.com]
Sent: Wednesday, August 08, 2001 9:15 AM
Subject: edge effects
OK a question for my own gratification. What causes the so called edge
i.e. increased none specific staining around the edge of sections, with IHC.
I have never seen the effect with IF. I know what everyone says, it's from
partial drying around the edge of sections. However I have seen it even in
sections completely flooded.
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