RE: edge effects

From:"Morken, Tim" <>

I think the "edge effect" is cause by drying/modifiying of the tissue
surface during sampling and processing than during staining. The staining
merely highlights it. Maybe it has to do with the relatively extreme
exposure the of outside surface of the tissue sample. The interior is much
less affected, I think.
Tim Morken

-----Original Message-----
From: []
Sent: Wednesday, August 08, 2001 9:15 AM
Subject: edge effects

OK a question for my own gratification. What causes the so called edge
i.e. increased none specific staining around the edge of sections, with IHC.

I have never seen the effect with IF. I know what everyone says, it's from 
partial drying around the edge of sections. However I have seen it even in 
sections completely flooded. 

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