RE: CK 5/6
|From:||"Auld, John" <John.Auld@rfh.nthames.nhs.uk>|
We use Dako CK 5/6 at 1/100 for one hour after 20 minutes microwave
in pH 6.0 Citrate buffer FOLLOWED by 15 secs trypsin digestion. This is the
retreival method we use for most of our cytokeratins. We get strong staining
with Vector elite universal and Vectors DAB. CK 5/6 as well as being useful
for mesothelioma's is good for basal cell staining in prostates, less
problematic (in our hands) than LP 34 or 34BE12.
> Date: 7 Aug 2001 11:53:25 -0500
> From: "Richard Cartun" <Rcartun@harthosp.org>
> Subject: Re: CK 5/6
> We use DAKO's CK5/6 at 1:100 overnight (evidently, not a very potent
> antibody!) with waterbath HIER. We use "tonsil" as our positive control.
> R. Cartun
> >>> THERESA ROHR <firstname.lastname@example.org> 08/06/01 02:19PM >>>
> I am again seeking help with a "new" antibody for my lab. Is anyone using
> DAKO CK 5/6 currently with Envision Plus on the Autostainer?
> If so, what dilution is working well for you? Also, what tissue would you
> suggest as a good control?
> It is great having access to your "minds" and also hearing from DAKO Reps.
> This site is saving me a lot of time and money by narrowing down my
> testing both in terms of time and reagents.
> Thanks in advance,
> Theresa Rohr BA HT(ASCP)
> Nyack Hospital
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