RE: ANTIGEN UNMASKING
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From: | "Weems, Joyce" <JWEEMS@sjha.org> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Date: | Fri, 2 Apr 1999 03:09:32 -0500 |
Content-Type: | text/plain; charset="us-ascii" |
Happy April Fool's everyone! j:>)
>----------
>From: Tim Morken[SMTP:timcdc@hotmail.com]
>Sent: Thursday, April 01, 1999 9:10 AM
>To: histonet@pathology.swmed.edu
>Subject: Re: ANTIGEN UNMASKING
>
>Mr. richards,
>
>Yes, we have done this and it works much better than any other heat
>retreival method. We have a Singer stream iron with 15 heat settings,
>but probably any would do. We got it at Wal-Mart. Of course you need a
>suitable surface to do the ironing and we found one in the bin out back
>- yes, a piece of cardboard is the best, and is perfectly recycleable.
>We generally iron for 6 minutes at setting 12, wool, I believe. One
>great benefit, besides the antigen unmasking, is the elimation of
>wrinkles.
>
>As an aside, we have an entire "kitchen" set up now, what with out
>microwave, steamer and pressure cooker. We are also using a Sears
>Hotpoint clothes dryer to dry slides. much cheaper than those sold in
>those expensive lab supply catalogs! We did have to disable the tumble
>function, however, as our slides got all mixed up and had to be
>re-sorted!
>
>
>Tim Morken, B.A., EMT(MSA), HTL(ASCP)
>Infectious Disease Pathology
>Centers for Cookery Control
>MS-G32
>1600 Clifton Rd.
>Atlanta, GA 30333
>USA
>
>email: tim9@cdc.gov
> timcdc@hotmail.com
>
>FAX: (404)639-3043
>
>
>
>----Original Message Follows----
>From: "M. Richards." <REE3@leicester.ac.uk>
>To: histonet@pathology.swmed.edu
>Subject: Re: ANTIGEN UNMASKING
>Date: Thu, 01 Apr 1999 08:31:00 +0100 (BST)
>
> Philips et al in a recent publication(J. Appl. Dom. Sci.
>Tech.1999;22,37-44)
>described the use of the domestic steam iron to achieve antigen
>unmasking in paraffin sections.The distilled water being replaced by
>citrate
>buffer pH6, and by 'ironing' the sections through absorbent brown
>paper
>the dewaxing step is combined with the antigen unmasking.
> I would be interested to hear from anyone
>who has had
>any success with this technique.
> Thanks in advance
>
> M. Richards........
>
>
>
>
>Get Your Private, Free Email at http://www.hotmail.com
>
>
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