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From:Patricia Karlisch <> (by way of histonet)
To:histonet <>
Date:Fri, 2 Apr 1999 03:09:33 -0500
Content-Type:text/plain; charset="us-ascii"

I can't believe I fell for this one.  I scratched my  head and said, geez,
I wonder how on earth this works.  Pat Karlisch
P.S. I hate to iron so this would never have worked anyway.

>>> Bonnie Whitaker <> 04/01 10:02 am >>>
Oh, to be so clever and original......Happy April Fool's Day, people.

-----Original Message-----
From: Tim Morken <>
To: <>
Date: Thursday, April 01, 1999 8:40 AM

>Mr. richards,
>Yes, we have done this and it works much better than any other heat
>retreival method. We have a Singer stream iron with 15 heat settings,
>but probably any would do. We got it at Wal-Mart. Of course you need a
>suitable surface to do the ironing and we found one in the bin out back
>- yes, a piece of cardboard is the best, and is perfectly recycleable.
>We generally iron for 6 minutes at setting 12, wool, I believe. One
>great benefit, besides the antigen unmasking, is the elimation of
>As an aside, we have an entire "kitchen" set up now, what with out
>microwave, steamer and pressure cooker. We are also using a Sears
>Hotpoint clothes dryer to dry slides. much cheaper than those sold in
>those expensive lab supply catalogs! We did have to disable the tumble
>function, however, as our slides got all mixed up and had to be
>Tim Morken, B.A., EMT(MSA), HTL(ASCP)
>Infectious Disease Pathology
>Centers for Cookery Control
>1600 Clifton Rd.
>Atlanta, GA 30333
>FAX:  (404)639-3043
>----Original Message Follows----
>From: "M.  Richards." <>
>Date: Thu, 01 Apr 1999 08:31:00 +0100 (BST)
>   Philips et al in a recent  publication(J. Appl. Dom.  Sci.
>described  the  use of  the  domestic  steam  iron  to  achieve  antigen
>unmasking in paraffin  sections.The distilled water being  replaced  by
>buffer  pH6, and  by 'ironing' the  sections  through absorbent  brown
>the  dewaxing  step  is  combined  with the  antigen  unmasking.
>                      I  would  be  interested to  hear  from anyone
>who has had
>any  success with this  technique.
>                                           Thanks  in  advance
>                                                M.  Richards........
>Get Your Private, Free Email at

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