RE: ANTIGEN UNMASKING

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From:Cindy Farman <cfarman@sierrabiomedical.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Date:Fri, 2 Apr 1999 03:09:32 -0500
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Did anyone see the movie Benny and Joon?

-----Original Message-----
From:	M.  Richards. [SMTP:REE3@leicester.ac.uk]
Sent:	Wednesday, March 31, 1999 11:31 PM
To:	histonet@Pathology.swmed.edu
Subject:	Re: ANTIGEN  UNMASKING



   Philips et al in a recent  publication(J. Appl. Dom.  Sci.
Tech.1999;22,37-44)
described  the  use of  the  domestic  steam  iron  to  achieve  antigen
unmasking in paraffin  sections.The distilled water being  replaced  by
citrate
buffer  pH6, and  by 'ironing' the  sections  through absorbent  brown  paper
the  dewaxing  step  is  combined  with the  antigen  unmasking.
                      I  would  be  interested to  hear  from anyone  who
has had
any  success with this  technique.
                                           Thanks  in  advance

                                                M.  Richards........
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