Sarah,
I haven't done too much of this combination, but it can definitely be
done. I would run your IHC first and then 'counterstain' with the
special stain of choice. We've used antibodies to pancreatic hormones
and then counterstained with PAS. The PAS does a better job of
outlining basement membranes as compared to a standard H&E. I'm not
sure if silver stains would be compatible, but I think there are
possibilities.
Tim Coskran
Pfizer
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