RE: [Histonet] Policy and procedures

From:"Tony Henwood"

We only include a note on a chemical used in a procedure if it is classified as hazardous (according to Criteria of Worksafe Australia). Eg:

>From Weigert's Iron Haematoxylin Procedure:
1.	10% Alcoholic Haematoxylin Warning: Flammable - see MSDS
2.	Aqueous Ferric Chloride Warning: Corrosive - see MSDS
		30% aqueous Ferric Chloride 		20ml
		Distilled water 			500ml
		Conc. Hydrochloric acid 		5ml

Whereas a solution such as 1% Periodic Acid, which is not classified as hazardous according to Criteria of Worksafe Australia does not have a warning in the procedure manual.

We have MSDSs (electronic)for all solutions prepared in our lab and it is from these that we determine the hazardous nature of the solution as well design the label. Each hazardous solution has a label with a watermark symbol to indicate its hazardous nature. This hopefully prevents the "familiarity breeds contempt" syndrome.

I suppose that every solution, if swallowed, is dangerous but our philosophy is to highlight only those solutions that are, at the present time, known to be hazardous.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: [] On Behalf Of Rene J Buesa
Sent: Friday, 27 April 2007 5:30 AM
To: Vacca Jessica;
Subject: Re: [Histonet] Policy and procedures

Since MSDS are available to all by just pulling the "3 rings binders" I did not include them in the SOP, neither the catalog numbers of the reagents since frequently we had to change vendors due to bulk contracts with the suppliers.
  I just included the procedure and a bibliographic reference for each (also my SOP was beautifully illustrated with photomicrographs of each procedure, it was gorgeous indeed!).
  René J.

Vacca Jessica  wrote:
  I was wondering how many of you include the product information and MSDS warnings in your policy and procedures on the chemicals that are used. I am reviewing my procedures and this information as well as the manufacturer and catalog numbers are included. I have to do some revisions as well incorporate new ones, and was wondering if all that information is actually required

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr.
Brandon Fl 33511
(813) 571-5193 or (813) 681-5551 ext 2454

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