RE: [Histonet] Use of 1% AgNo3 in gross room

From:Bryan Llewellyn

I worked in a place where they did this with small breast biopsies.  The 
silver nitrate solution gets all dark and sludgy from tissue chemicals and 
the formalin.  It stops working after a short time.  Very expensive and not 
worth the trouble.  Use tattoo inks, much cheaper, safer, prettier, and you 
can mark each margin a different colour.

Bryan Llewellyn

----- Original Message ----- 
From: "Greg Dobbin" 
Sent: Thursday, April 26, 2007 7:18 AM
Subject: [Histonet] Use of 1% AgNO3 in gross room

> Hi Folks,
> In our gross room, (and maybe in most-I'm not familiar with the
>    practise) our pathologists are using 1% silver nitrate to see
> tumor
>    margins in bowels, breast, etc. by pouring the solution onto the
> tissue
>    prior to slicing.  One pathologist has suggested keeping a bucket
> of 1%
>    AgNO3 in the gross room so that the tissue could instead be
> briefly
>    immersed.  I know that if we use a plastic bucket the plastic will
> be
>    forever stained which is of course no big deal, but will the
> plastic
>    compromise the shelf life of the solution itself?
>    Thanks for your time.
>    Greg
> Greg Dobbin, R.T.
> Chief Technologist, Histology Lab
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE    C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
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