RE: Daily Digest
I am looking for commercial software package for tracking of submissions to a
histology laboratory. Please let me know if there are any in existance. Thanks
Ron
> ----------
> From: HistoNet Server
> Sent: TuesdayWedApril 23, 2002 1:00 AM
> To: HistoNet Server
> Subject: Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 02:45:27 -0500
> From: Kappeler Andi
> Subject: Re: Immunotech antibodies
>
> Immunotech once was a French company, was then bought by Coulter, which is
> now Beckman-Coulter (next year: ???). However, the antibodies originally
> marketed by Immunotech do still exist and can be identified in the Beckman
> Coulter catalogue as such, because their cat.nr. always starts with 'IM...'.
> More info at http://www.beckmancoulter.com/products/pr_immunology.asp. Hope
> this helps.
> Andi
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
>
> - -----Ursprungliche Nachricht-----
> Von: Klassen, Shannon SDH
> An: 'Histonet'
> Gesendet: Donnerstag, 18. April 2002 21:33
> Betreff: Immunotech antibodies
>
>
> > There used to be a line of antibodies from a company called Immunotech -
> > does it still exist? Website?
> > Shannon Klassen
> > IHC Tech
> > Saskatoon Health District
> > Saskatoon, Canada
> >
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 07:45:25 -0500
> From: ajennings@unmc.edu
> Subject: Charcot-Leyden crystals-birefringence?
>
>
> Not sure if birefringence is appropriate description, or if it is just my
> definition of "birefringment". the crystals that we observed were opague
> and stained red, not what I considered "normal" crystal appearance. anyone
> have any help with this? I know the dictionary definition and I dont want
> to get into index of refraction etc. (or I could ask on the confocal
> server) I just want clarification of the term when is comes to
> observing crystals under the scope and a description of what is observed.
> i.e. transparent etc.
> thanks
> anita
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 08:01:00 -0500
> From: "Morken, Tim"
> Subject: Histonet: Any US state health department histology people out the
> re?
>
> If you work in a histology/immunohistochemistry lab in a US state health
> department, please contact me privately.
>
> Tim Morken
> CDC, Atlanta
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 08:04:35 -0500
> From: Bernard Ian R SSgt
> Subject: RE: Undecalcified bone
>
> Hello Ernie,
> I myself work with processing and cutting undecalcified bone
> in plastics using the Exakt and Microm HM 355 S.
> I will like to get as much info on Plastic processing, microtomy techniques
> and the work fo bone tissue.
>
>
> Ian R. Bernard, SSgt, USAF, AAS, HT (ASCP)
> NCOIC Histopathology/ Pathobiology Laboratory
> 59th Clinical Research Sqd / MSROP
> 1255 Wilford Hall Loop, bldg 4430
> Lackland AFB, Tx 78236-5319
>
> Tel: 210-292-7190
> Fax: 210-292-6053
> E-mail: Ian.bernard@59mdw.whmc.af.mil
>
>
>
> - -----Original Message-----
> From: ErnieMiddleton@aol.com [mailto:ErnieMiddleton@aol.com]
> Sent: Sunday, April 21, 2002 7:33 PM
> To: histonet@pathology.swmed.edu
> Subject: Undecalcified bone
>
>
> Hi,
> A few weeks ago, two individuals promised to help me with tips on
> undecalcified bone procedures. Please e-mail me and give me your e-mail
> address. Thank in advance for your help.
>
> Ernestine Middleton HT/ HTL
> Montefiore Med. Ct.
> University Hospital of Albert Einstein
> College of Medicine
> Bronx, NY
> ErnieMiddleton@aol.com
> Fax 718-547-1920
> 718-920-4157
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 09:00:49 -0500
> From: Hewlett Bryan
> Subject: RE: Charcot-Leyden crystals-birefringence?
>
>
> Anita,
>
> These acidophilic needle or lance shaped crystals will appear red and
> slightly opaque on H&E stains examined by brightfield microscopy.
> Perhaps I should have said that these crystals exhibit anisotropism!
> The terms anisotropic and birefringent are commonly interchanged.
> Birefringence is observed by means of a polarizing microscope.
> With crossed polarizers certain objects, such as Charcot-Leyden crystals,
> will exhibit anisotropism/birefringence and will appear bright on a dark
> background. When the specimen is rotated these objects will blink on and off
> approximately every 90 degrees.
> When a birefringent object is transilluminated by plane polarized light,
> each light ray is split into two part-rays. The ordinary ray passes through
> the object in a straight line(i.e. as in isotropic objects), whereas the
> second ray is laterally displaced(i.e. as in anisotropic objects). These two
> part rays differ in polarization direction by 90 degrees. They also differ
> in their velocity of propogation. That is, they have different refractive
> indices, hence the object producing them is birefringent.
>
> Bryan
> > ----------
> > From: ajennings@unmc.edu[SMTP:ajennings@unmc.edu]
> > Sent: April 22, 2002 7:31 AM
> > Cc: Histonet@pathology.swmed.edu
> > Subject: Charcot-Leyden crystals-birefringence?
> >
> >
> > Not sure if birefringence is appropriate description, or if it is just my
> > definition of "birefringment". the crystals that we observed were opague
> > and stained red, not what I considered "normal" crystal appearance. anyone
> > have any help with this? I know the dictionary definition and I dont want
> > to get into index of refraction etc. (or I could ask on the confocal
> > server) I just want clarification of the term when is comes to
> > observing crystals under the scope and a description of what is observed.
> > i.e. transparent etc.
> > thanks
> > anita
> >
> >
> >
> This information is directed in confidence solely to the person named above
> and may not otherwise be distributed, copied or disclosed. Therefore, this
> information should be considered strictly confidential. If you have
> received this email in error, please notify the sender immediately via a
> return email for further direction. Thank you for your assistance.
>
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This message is in MIME format. Since your mail reader does not understand
> this format, some or all of this message may not be legible.
>
> - --Boundary_(ID_HHsRT6jJqf8GFfNbstfgiQ)
> Content-type: text/plain
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_HHsRT6jJqf8GFfNbstfgiQ)
> Content-type: text/html
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
> RE: Charcot-Leyden crystals-birefringence?
>
>
>
> Anita,
>
>
> These acidophilic needle or
> lance shaped crystals will appear red and slightly opaque on H&E stains
> examined by brightfield microscopy.
>
> Perhaps I should have said
> that these crystals exhibit anisotropism!
>
The terms anisotropic and
> birefringent are commonly interchanged.
>
Birefringence is observed by
> means of a polarizing microscope.
>
With crossed polarizers
> certain objects, such as Charcot-Leyden crystals, will exhibit
> anisotropism/birefringence and will appear bright on a dark background. When
> the specimen is rotated these objects will blink on and off approximately
> every 90 degrees.
>
> When a birefringent object is
> transilluminated by plane polarized light, each light ray is split into two
> part-rays. The ordinary ray passes through the object in a straight line(i.e.
> as in isotropic objects), whereas the second ray is laterally displaced(i.e.
> as in anisotropic objects). These two part rays differ in polarization
> direction by 90 degrees. They also differ in their velocity of propogation.
> That is, they have different refractive indices, hence the object producing
> them is birefringent.
>
> Bryan
>
> ----------
>
From: FACE="MS Sans Serif">ajennings@unmc.edu[SMTP:ajennings@unmc.edu]
>
Sent: FACE="MS Sans Serif">April 22, 2002 7:31 AM
>
Cc:
> Histonet@pathology.swmed.edu
>
Subject:
> Not sure if birefringence is appropriate
> description, or if it is just my
>
definition of "birefringment". the
> crystals that we observed were opague
>
and stained red, not what I considered
> "normal" crystal appearance. anyone
>
have any help with this? I know the dictionary
> definition and I dont want
>
to get into index of refraction etc. (or I could
> ask on the confocal
>
server) <smile> I just want clarification
> of the term when is comes to
>
observing crystals under the scope and a
> description of what is observed.
>
i.e. transparent etc.
>
thanks
>
anita
>
>
>
>
> This information is directed in confidence
> solely to the person named above and may not otherwise be distributed, copied
> or disclosed. Therefore, this information should be considered strictly
> confidential. If you have received this email in error, please notify
> the sender immediately via a return email for further direction. Thank you for
> your assistance.
>
>
>
>
>
> - --Boundary_(ID_HHsRT6jJqf8GFfNbstfgiQ)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 09:45:35 -0500
> From: "Patterson, Noelle (NIDDK)"
> Subject: Histonet archives/section whole mouse pancreas
>
> Hello. Can someone send along instructions to access Histonet archives? I
> am looking into sectioning whole mouse organs; a topic discussed previously.
> Just wanted to get some pointers and what is needed to get started. In
> particular we want to section the whole length of the pancreas (yes, laid
> out as close "in vivo" as possible).
>
> Previous discussions have been about whole rat brain; that should help.
>
> Thanks,
> Noelle
>
> Noelle Patterson
> Biologist
> NIDDK/Navy-TAB
> Bethesda, MD 20889-5603
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 10:30:48 -0500
> From: "Charles W. Scouten, Ph.D."
> Subject: RE: Histonet archives/section whole mouse pancreas
>
> Go to Histonet.org, the new picture site. From there, there is a link
> to the histonet archives.
>
> Cordially,
>
> Charles W. Scouten, Ph.D.
> myNeuroLab.com
> 5918 Evergreen Blvd.
> St. Louis, MO 63134
> Ph: 314 522 0300
> FAX 314 522 0277
> cwscouten@myneurolab.com
> www.myneurolab.com
>
>
> - -----Original Message-----
> From: Patterson, Noelle (NIDDK) [mailto:NoelleP@intra.niddk.nih.gov]
> Sent: Monday, April 22, 2002 9:31 AM
> To: Histonet (E-mail)
> Subject: Histonet archives/section whole mouse pancreas
>
> Hello. Can someone send along instructions to access Histonet archives?
> I
> am looking into sectioning whole mouse organs; a topic discussed
> previously.
> Just wanted to get some pointers and what is needed to get started. In
> particular we want to section the whole length of the pancreas (yes,
> laid
> out as close "in vivo" as possible).
>
> Previous discussions have been about whole rat brain; that should help.
>
> Thanks,
> Noelle
>
> Noelle Patterson
> Biologist
> NIDDK/Navy-TAB
> Bethesda, MD 20889-5603
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 10:31:13 -0500
> From: "Patterson, Noelle (NIDDK)"
> Subject: RE: Histonet archives/section whole mouse pancreas
>
> Thanks!
>
> - -----Original Message-----
> From: Charles W. Scouten, Ph.D. [mailto:cwscouten@myneurolab.com]
> Sent: Monday, April 22, 2002 11:10 AM
> To: Patterson, Noelle (NIDDK); Histonet (E-mail)
> Subject: RE: Histonet archives/section whole mouse pancreas
>
>
> Go to Histonet.org, the new picture site. From there, there is a link
> to the histonet archives.
>
> Cordially,
>
> Charles W. Scouten, Ph.D.
> myNeuroLab.com
> 5918 Evergreen Blvd.
> St. Louis, MO 63134
> Ph: 314 522 0300
> FAX 314 522 0277
> cwscouten@myneurolab.com
> www.myneurolab.com
>
>
> - -----Original Message-----
> From: Patterson, Noelle (NIDDK) [mailto:NoelleP@intra.niddk.nih.gov]
> Sent: Monday, April 22, 2002 9:31 AM
> To: Histonet (E-mail)
> Subject: Histonet archives/section whole mouse pancreas
>
> Hello. Can someone send along instructions to access Histonet archives?
> I
> am looking into sectioning whole mouse organs; a topic discussed
> previously.
> Just wanted to get some pointers and what is needed to get started. In
> particular we want to section the whole length of the pancreas (yes,
> laid
> out as close "in vivo" as possible).
>
> Previous discussions have been about whole rat brain; that should help.
>
> Thanks,
> Noelle
>
> Noelle Patterson
> Biologist
> NIDDK/Navy-TAB
> Bethesda, MD 20889-5603
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 10:31:30 -0500
> From: c.mackin@biogenex.com
> Subject: unsuscribe
>
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This message is in MIME format. Since your mail reader does not understand
> this format, some or all of this message may not be legible.
>
> - --Boundary_(ID_40JUzNHYGpby5LdB8Dmb3A)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body (Could be empty)" >>>>>>
>
> - --Boundary_(ID_40JUzNHYGpby5LdB8Dmb3A)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
> unsuscribe
>
>
>
>
>
>
> - --Boundary_(ID_40JUzNHYGpby5LdB8Dmb3A)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 10:31:50 -0500
> From: Tom McNemar
> Subject: RE: c-kit (CD117)
>
>
> Same here.
>
> Tom Mc Nemar HT(ASCP)
> Histology Supervisor
> Licking Memorial Hospital
> Newark, Ohio
>
> - -----Original Message-----
> From: eileen dusek [mailto:eileen_dusek@hotmail.com]
> Sent: Thursday, April 18, 2002 12:59 PM
> To: klassens@sdh.sk.ca; histonet@pathology.swmed.edu
> Subject: Re: c-kit (CD117)
>
>
>
> We have been successful with Dako's CD117 using it at 1:100 with out any
> treatment.
>
> Our control tissue is a gastrointestinal stromal tumor.
>
> I hope this helps.
>
> Eileen C. Dusek
>
> Central DuPage Hospital
>
>
>
> >From: "Klassen, Shannon SDH"
> >To: 'Histonet'
> >Subject: c-kit (CD117)
> >Date: Thu, 18 Apr 2002 09:55:52 -0600
> >
> >Can someone out there in histoland help me get this antibody working -
> >Novocastra ckit clone T595 - I'm not getting membrane staining - some
> >cytoplasmic and some almost appears nuclear. I've tried a variety of
> >different tumors - GIST, melanoma, breast , normal skin and haven't had any
>
> >success.
> >Down to 1\10 with antigen retrieval in citrate buffer in microwave.
> >HEELLLPPP!!
> >
> >Shannon Klassen
> >IHC Tech
> >Saskatoon Health District
> >Saskatoon, Canada
> >
>
> _____
>
> Get your FREE download of MSN Explorer at http://explorer.msn.com
> .
>
>
>
>
>
>
> The Information contained in this e-mail message is privileged and
> confidential, and is intended for the use of the addressee and no one else.
> If you are not the intended recipient, please do not read or use this e-mail
> message and notify the sender of the mistaken transmission.
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This message is in MIME format. Since your mail reader does not understand
> this format, some or all of this message may not be legible.
>
> - --Boundary_(ID_xrIml1bEYo34/MIkQsua6Q)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_xrIml1bEYo34/MIkQsua6Q)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Same
>
> here.
> class=590123115-22042002>
>
> Tom Mc Nemar HT(ASCP)
size=2>Histology Supervisor
Licking
> Memorial
> Hospital
Newark, Ohio
>
>
>
>
>
>
We have been successful with Dako's CD117 using it at 1:100 with out any
> treatment.
>
Our control tissue is a gastrointestinal stromal tumor.
>
I hope this helps.
>
Eileen C. Dusek
>
Central DuPage Hospital
>
>
>From: "Klassen, Shannon SDH"
> >To: 'Histonet'
> >Subject: c-kit (CD117)
> >Date: Thu, 18 Apr 2002 09:55:52 -0600
> >
> >Can someone out there in histoland help me get this antibody
> working -
> >Novocastra ckit clone T595 - I'm not getting membrane
> staining
> - some
> >cytoplasmic and some almost appears nuclear. I've tried a
> variety of
> >different tumors - GIST, melanoma, breast , normal skin and
> haven't had any
> >success.
> >Down to 1\10 with antigen retrieval in citrate buffer in
> microwave.
> >HEELLLPPP!!
> >
> >Shannon Klassen
> >IHC Tech
> >Saskatoon Health District
> >Saskatoon, Canada
> >
>
>
> Get your FREE download of MSN Explorer at
> href="http://g.msn.com/1HM205401/i">http://explorer.msn.com.
TE>
>
>
>
>
>
> The Information contained in this e-mail message
> is privileged and confidential, and is intended for the use of the addressee
> and no one else. If you are not the intended recipient, please do not read or
> use this e-mail message and notify the sender of the mistaken
> transmission.
>
> - --Boundary_(ID_xrIml1bEYo34/MIkQsua6Q)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 11:31:35 -0500
> From: Shirley Powell
> Subject: whole section followup
>
> Hi All,
>
> Back a couple of weeks or so someone requested written procedures on
> processing whole organ sections. I provided my procedure for this. But
> at the time, I forgot there is also an article in the JOH, volume 6, No
> 1, March 1983 written by Ernestene Sims which gives her procedure for
> whole organ processing (brain) done on the VIP.
>
> Shirley
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 14:45:47 -0500
> From: mward@wfubmc.edu (Martha Ward)
> Subject: HIV + frozen sections
>
> Martha Ward writes:
>
> This question is for folks who are doing direct immunofluorescence on
> renal biopsies. How do you handle decontamintion of the cryostat after
> cutting a known HIV + patient? Our current procedure is to break down
> the cryostat, wipe down with alcohol, emerse the microtome in 100%
> alcohol, soak for 1 hour, allow it to dry overnight in a 40 C incubator
> and put everything back to together the next day. Is there a better way
> of doing this, or is this the way most people handle this? This
> question would also be for routine maintenance of the cryostat, not just
> when we get known infectious sample.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 14:46:07 -0500
> From: Tara Miller
> Subject: RE: RETRIEVAL USING PRESSURE COOKER
>
> Amy,
>
> We use steam monitor strips from Biocare Medical, cat#613.
> They come in little zip lock bags of 25, and we just lay one across the top
> of one of our coplin jars before closing the pressure cooker.
> Biocare Medical - (800)799-9499
>
> Tara
>
>
> >From: Amy Self
> >To: "'Tara Miller'"
> >Subject: RE: RETRIEVAL USING PRESSURE COOKER
> >Date: Fri, 19 Apr 2002 12:40:43 -0400
> >
> >
> > Tara,
> > We do a few immunos in our small lab using the black and decker
> >steamer - was wandering where do you get the steamer strips from and how
> >are
> >you using these strips? Thanks
> >
> >
> > Amy
> >
> >-----Original Message-----
> >From: Tara Miller [mailto:taratheht@hotmail.com]
> >Sent: Friday, April 19, 2002 11:23 AM
> >To: histonet@pathology.swmed.edu
> >Subject: Re: RETRIEVAL USING PRESSURE COOKER
> >
> >
> >At first we were marking on sheets (one for each pH) that our pH strips had
> >detected the correct pH. However now we save all the strips, both steam
> >strips and the pH strips, by just taping them all into a log book. Of
> >course
> >
> >we first let them dry out and the pH strips fade a little, but it
> >demonstrates that we did the necessary testing. Also, we log the date and
> >whether the steam strip was used in the pressure cooker or the steamer.
> >And,
> >
> >if we do more than on run we mark 1st run and 2nd run next to the strips.
> >
> >Tara Oakes, H.T.
> >Central DuPage Hospital
> >
> >
> > >From: "Nava, Josefa"
> > >To: "'HISTONET@PATHOLOGY.SWMED.EDU.'"
> > >Subject: RETRIEVAL USING PRESSURE COOKER
> > >Date: Thu, 18 Apr 2002 13:55:48 -0500
> > >
> > >I am curious how everyone is doing their QC on the IHC pressure cooker.
> > >Do
> > >you have a form to log in your temperature and pressure evrytime you
> >run
> > >your pressure cooker everyday? For those who are using the Biocare
> >pressure
> > >cooker what type of Quality control do
> > >you have aside from using the Monitor strips. And do you have an everyday
> > >log to record your reaction on your monitor strips or do you even save
> > >those strips for CAP inspection? I appreciate any feedback you can give
> > >me
> > >on this matter.
> > >
> > >Josie
> > >
> >
> >
> >_________________________________________________________________
> >MSN Photos is the easiest way to share and print your photos:
> >http://photos.msn.com/support/worldwide.aspx
> >
> >
> >
> >Note: The information contained in this message may be privileged and
> >confidential and protected from disclosure. If the reader of this message
> >is
> >not the intended recipient, or an employee or agent responsible for
> >delivering this message to the intended recipient, you are hereby notified
> >that any dissemination, distribution or copying of this communication is
> >strictly prohibited. If you have received this communication in error,
> >please notify us immediately by replying to the message and deleting it
> >from
> >your computer. Thank you.
> >
> >
>
>
> _________________________________________________________________
> Send and receive Hotmail on your mobile device: http://mobile.msn.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 15:15:47 -0500
> From: Amy Porter
> Subject: Need help for decal samples
>
>
> We are working on a research project involving mouse bone. Trying to perform
> Luna's stain for eosinophils. Tissues have been decaled with formic for a
> predetermined length of time by the researcher, then rinsed in running water
> for 4 hours and processed by standard methods. The stain seems to be
> diffusely staining no matter the variations that we try, without much luck
> seeing the eosinophils. All samples that are formalin fixed without decal are
> staining beautifully. Any suggestions would be helpful.
>
> Amy S.Porter, HT(ASCP)
> Michigan State University
> College of Human Medicine
> portera203@yahoo.com
>
>
> - ---------------------------------
> Do You Yahoo!?
> Yahoo! Games - play chess, backgammon, pool and more
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
>
> - --Boundary_(ID_Wf0gjzehL0xdZCmvszlfwQ)
> Content-type: text/plain; charset=us-ascii
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_Wf0gjzehL0xdZCmvszlfwQ)
> Content-type: text/html; charset=us-ascii
> Content-transfer-encoding: 7BIT
>
> We are working on a research project involving mouse bone. Trying to
> perform Luna's stain for eosinophils. Tissues have been decaled with
> formic for a predetermined length of time by the researcher, then rinsed in
> running water for 4 hours and processed by standard methods. The stain
> seems to be diffusely staining no matter the variations that we try, without
> much luck seeing the eosinophils. All samples that are formalin fixed
> without decal are staining beautifully. Any suggestions would be
> helpful.
Amy S.Porter, HT(ASCP)
Michigan State University
College
> of Human Medicine
portera203@yahoo.com
Do You
> Yahoo!?
> Yahoo! Games - play
> chess, backgammon, pool and more
>
> - --Boundary_(ID_Wf0gjzehL0xdZCmvszlfwQ)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 15:16:02 -0500
> From: Jeff Gordon
> Subject: RE: RETRIEVAL USING PRESSURE COOKER
>
> If you are using the Trilogy/Declere standardized pressure cooker
> pretreatment protocol, there are autoclave strips available through
> Fisher that can also be used for internal monitoring.
>
> Jeff Gordon
> Cell Marque Corp.
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 15:16:19 -0500
> From: "Charles.Embrey"
> Subject: RE: HIV + frozen sections
>
> I don't think the HIV virus itself would be a problem since the cold of the
> cryostat would kill it very quickly.
> Chuck
>
> - -----Original Message-----
> From: mward@wfubmc.edu [mailto:mward@wfubmc.edu]
> Sent: Monday, April 22, 2002 2:34 PM
> To: histonet@pathology.swmed.edu
> Subject: HIV + frozen sections
>
>
> Martha Ward writes:
>
> This question is for folks who are doing direct immunofluorescence on
> renal biopsies. How do you handle decontamintion of the cryostat after
> cutting a known HIV + patient? Our current procedure is to break down
> the cryostat, wipe down with alcohol, emerse the microtome in 100%
> alcohol, soak for 1 hour, allow it to dry overnight in a 40 C incubator
> and put everything back to together the next day. Is there a better way
> of doing this, or is this the way most people handle this? This
> question would also be for routine maintenance of the cryostat, not just
> when we get known infectious sample.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 15:45:20 -0500
> From: MadCityKenners@aol.com
> Subject: half-price!
>
> Greetings histonetters!
>
> I have a laboratory microwave for sale (Model H2300) ~ price new is
> $4000, am asking $2000. It is time/temperature controlled, and takes up very
> little space in your lab!
>
> Rita Kenner HTL
> St Joseph Community Hospital
> West Bend, WI
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 16:00:47 -0500
> From: Serena Leung
> Subject: reference request
>
> Hi!
>
> Does anyone have a copy of the following article that they could send me?
>
> Martin, Lynn, Nickey, "A Rapid Polychrome Stain for Epoxy Embedded Tissue", Am
> J Clin Path 46:250,1966
>
> Thanks in advance!
> Serena Leung
> Anderson Orthopaedic Research Institute
> SLeung@aori.org
> phone: 703-619-4414
> fax: 703-799-5982
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 16:01:03 -0500
> From: DeBari@mail.holyname.org
> Subject: RE: sakura tissue TEK coverslipper
>
>
> I'm seeking advice/comments from labs using the sakura tissue TEK
> coverslipper. Are you having any problems with instrument? Do you have an
> additional fume hood for the xylene odor or is the attached fumigation
> system sufficient?
> While the histology department is having great results, cytology slides
> have a bite of cornflaking. Any suggestions on how to fix this?
> Thanks
> Christina
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 16:07:44 -0500
> From: Michelle Rederick
> Subject: Job opening
>
> I have a position open for a histotech on the evening shift (start time
> flexible 8pm-11pm) at the Ohio State University Medical Center in Columbus,
> OH. The starting wage is between $12-15 depending on experience. We offer
> excellent benefits. We process 56,000 cases per year and have 17 FT techs.
>
> The Ohio State University Medical Center is a partnership between The Ohio
> State University Hospitals, OSU Hospitals East, The Arthur G. James Cancer
> Hospital and Research Institute and The Ohio State University College of
> Medicine and Public Health. The mission of the Medical Center includes three
> elements - patient care, teaching and research. This three-part mission, and
> a staff dedicated to its fulfillment, distinguishes The Ohio State
> University Medical Center as Ohio's premier medical center. More than 1,100
> medical staff and 4,200 support staff members provide care to 33,000
> inpatients and 625,000 outpatients every year.
> Maintaining a balance between routine and advanced care, University Medical
> Center is firmly established in a select group of outstanding medical
> institutions. Hospitals throughout the country look to The Ohio State
> University Medical Center for the latest advances in patient care, research
> and technology.
>
> Interested applicants should contact me by e-mail at: MRederick@neo.rr.com
>
> Thank you,
>
> Michelle Rederick HTL(ASCP) Histology Lab Manager
> OSU Medical Center Columbus, OH
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 16:31:18 -0500
> From: ANN MARUSKA
> Subject: p24
>
>
> Hi there,
>
> Anyone out there is Histoland doing an IHC stain for HIV using antibody p24
> from Dako?
> I have been trying to work it up on on tissue fixed in Shreks and processed
> for a researcher. There are plasma cells present - but the specimen came from
> some 3rd world country and has set in this fixative for a few weeks.
> .......so far, no staining. Have not been able to determine if it is my
> tissue or protocol that is giving me the problem......all suggestions welcome!
>
> Ann
>
> Ann Maruska
> Fairview-University Medical Center
> Mpls. MN 55454
> amarusk1@fairview.org
> 612-273-9119
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a MIME message. If you are reading this text, you may want to
> consider changing to a mail reader or gateway that understands how to
> properly handle MIME multipart messages.
>
> - --Boundary_(ID_qa15ftTTOJE09pB+rvalRA)
> Content-type: text/plain; charset=US-ASCII
> Content-transfer-encoding: 7BIT
> Content-disposition: inline
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_qa15ftTTOJE09pB+rvalRA)
> Content-type: text/plain; NAME="ANN MARUSKA.vcf"
> Content-transfer-encoding: 7BIT
> Content-disposition: attachment; filename="ANN MARUSKA.vcf"
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:MARUSKA, ANN
> EMAIL;WORK;PREF;NGW:AMARUSK1.EAST.NORTH
> X-GWUSERID:AMARUSK1
> ORG:;LAB
> N:MARUSKA;ANN
> TEL;WORK:612-273-9119
> END:VCARD
>
>
> - --Boundary_(ID_qa15ftTTOJE09pB+rvalRA)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 16:45:31 -0500
> From: "Donato, Elizabeth M"
> Subject: RE: Daily Digest
>
> To all HistoNet members and their colleagues,
>
> I am writing to post an add for a histology technician opening we have here
> at the Fred Hutchinson Cancer Research Center in Seattle, WA. This is a
> unique opportunity for an experienced histology technician to support
> research efforts in a non-clinical, challenging work setting. We are
> looking for an individual to consult with researchers in grant preparation,
> adapt new histology techniques to meet the needs of research projects,
> optimize existing techniques, assist with design of protocols and perform
> sectioning and H&E staining for immunohistochemistry and molecular
> analysis. Please see
> http://www.fhcrc.org/admin/hr/jobbullet/research_laboratory/HistologyTechKSW
> 13712.htm for a more detailed job description and feel free to contact me if
> you have any questions.
>
> Regards,
>
> Liz Donato
> Fred Hutchinson Cancer Research Center
>
> edonato@fhcrc.org
> 206.667.4501
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 17:30:17 -0500
> From: Richard Cartun
> Subject: Re: p24
>
> We use DAKO's anti-p24 HIV mAb with their EnVision+ detection and AEC
> chromogen. In the past we used enzyme (pepsin) digestion, but more
> recently we have seen excellent results with HIER (96 degree waterbath)
> pretreatment. What are you using for a positive control?
>
> R. Cartun
>
> >>> ANN MARUSKA 04/22/02 06:24PM >>>
> Hi there,
>
> Anyone out there is Histoland doing an IHC stain for HIV using antibody
> p24 from Dako?
> I have been trying to work it up on on tissue fixed in Shreks and
> processed for a researcher. There are plasma cells present - but the
> specimen came from some 3rd world country and has set in this fixative
> for a few weeks.
> .......so far, no staining. Have not been able to determine if it is
> my tissue or protocol that is giving me the problem......all suggestions
> welcome!
>
> Ann
>
> Ann Maruska
> Fairview-University Medical Center
> Mpls. MN 55454
> amarusk1@fairview.org
> 612-273-9119
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 18:30:41 -0500
> From: "Connie P."
> Subject: Breast specimens
>
>
> Could we please revisit my current nemesis, which is surely going to drive me
> into leaving Histology forever and seeking a lucrative highly paying
> successful career?
> I need help/advice/suggestions regarding handling breast specimens so that
> they are well preserved, easy to cut on the microtome, instead of partially
> raw tissue which requires the cut and scoop method to obtain a slide.
> I plead with the residents (1st yrs.) to trim them so that they fit in the
> cassette without touching the sides, and cut them to 2mm in thickness. Of
> course, most times they are 4mm or more. They are then placed into cassettes
> and held overnight in 10%NBF. They get put on the processor the next evening.
> I tried alcoholic formalin, saw no difference. Large breast lymph nodes are
> just as bad if not worse, raw in the centers. The small ones are usually fixed
> O.K. Does anyone have a tried and true method for handling large breast
> specimens which works for them with few exceptions? If so, please share it, I
> will be forever in your debt.
> Connie L. Probert
> Detroit, Michigan
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_lD0Mj8LycalMJrtgamkbBQ)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_lD0Mj8LycalMJrtgamkbBQ)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Could we please revisit my current nemesis, which
>
> is surely going to drive me into leaving Histology forever and seeking a
> lucrative highly paying successful career?
> I need
> help/advice/suggestions regarding handling breast specimens so that
> they are well preserved, easy to cut on the microtome, instead of partially
> raw
> tissue which requires the cut and scoop method to obtain a slide.
>
> I plead with the residents (1st yrs.) to trim
> them
> so that they fit in the cassette without touching the sides, and cut them to
> 2mm
> in thickness. Of course, most times they are 4mm or more. They are then placed
>
> into cassettes and held overnight in 10%NBF. They get put on the processor the
>
> next evening. I tried alcoholic formalin, saw no difference. Large breast
> lymph
> nodes are just as bad if not worse, raw in the centers. The small ones are
> usually fixed O.K. Does anyone have a tried and true method for handling large
>
> breast specimens which works for them with few exceptions? If so, please share
>
> it, I will be forever in your debt.
> Connie L. Probert
> Detroit, Michigan
>
> - --Boundary_(ID_lD0Mj8LycalMJrtgamkbBQ)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 19:46:09 -0500
> From: Aidan Schurr
> Subject: Re: Breast specimens
>
> Hi Connie,
>
> Unfortunately there is no "wonder solution" that will deal with tissue that
> has been cut too thick. This is because the tissue presses up against the
> inside surfaces of the cassette. This means the fluids (no matter what they
> are) cannot infiltrate properly, leading to poorly processed tissue. It's a
> matter of educating those Path's!! My solution to this has been twofold: 1 -
> watch them at cutup and ask them to thin down any tissue greater than 3mm
> thickness. It does help if you tell them exactly *why* you need the tissue to
> be thinner. Constant hounding does work eventually! 2 - get them in to try
> to cut the section the next day. Again, tell them why the tissue is that
> difficult to cut. Maybe compare to a "well processed" block of fat. There
> seems to be this subconscious idea that it is something we do, or some fault
> with the processing cycle that causes these difficulties. It's not easy, but
> you can change that mentality!
>
> There is no good reason that fresh breast tissue cut 2mm thick should not be
> perfectly sectionable after a 14hr overnight process!
>
> regards (and best wishes!)
>
> Aidan.
>
>
> __
>
> aidan schurr b.m.l.sc
> section head, histology
> hutt valley district health board
> lower hutt
> new zealand
>
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173 (direct)
> ++64 4 570 9214 (fax)
>
> >>> "Connie P." 23/04/2002 >>>
> Could we please revisit my current nemesis, which is surely going to drive me
> into leaving Histology forever and seeking a lucrative highly paying
> successful career?
> I need help/advice/suggestions regarding handling breast specimens so that
> they are well preserved, easy to cut on the microtome, instead of partially
> raw tissue which requires the cut and scoop method to obtain a slide.
> I plead with the residents (1st yrs.) to trim them so that they fit in the
> cassette without touching the sides, and cut them to 2mm in thickness. Of
> course, most times they are 4mm or more. They are then placed into cassettes
> and held overnight in 10%NBF. They get put on the processor the next evening.
> I tried alcoholic formalin, saw no difference. Large breast lymph nodes are
> just as bad if not worse, raw in the centers. The small ones are usually fixed
> O.K. Does anyone have a tried and true method for handling large breast
> specimens which works for them with few exceptions? If so, please share it, I
> will be forever in your debt.
> Connie L. Probert
> Detroit, Michigan
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 19:49:59 -0500
> From: Melissa Jensen
> Subject: Re: Breast specimens
>
>
> Ah.The old stew meat vs. lean cuisine!..When we get spec.that are
> unfixed..like the ones you have..we run them back thru the processor on clean
> cycle..than return to formalin..and put back on processor with the normal
> run.I realize it delays results a day..But hey..They cant read what they
> submitted anyway..you could try 70% alcohol ..let the fatty tissue sit in it
> until processing. You will just have to let the new residents know..if you do
> this..to big ect..that's fine..but you wont have results for a couple
> days.Good Luck!
> ----- Original Message -----
> From: Connie P.
> To: Histonet
> Sent: Monday, April 22, 2002 6:16 PM
> Subject: Breast specimens
>
>
> Could we please revisit my current nemesis, which is surely going to drive
> me into leaving Histology forever and seeking a lucrative highly paying
> successful career?
> I need help/advice/suggestions regarding handling breast specimens so that
> they are well preserved, easy to cut on the microtome, instead of partially
> raw tissue which requires the cut and scoop method to obtain a slide.
> I plead with the residents (1st yrs.) to trim them so that they fit in the
> cassette without touching the sides, and cut them to 2mm in thickness. Of
> course, most times they are 4mm or more. They are then placed into cassettes
> and held overnight in 10%NBF. They get put on the processor the next evening.
> I tried alcoholic formalin, saw no difference. Large breast lymph nodes are
> just as bad if not worse, raw in the centers. The small ones are usually fixed
> O.K. Does anyone have a tried and true method for handling large breast
> specimens which works for them with few exceptions? If so, please share it, I
> will be forever in your debt.
> Connie L. Probert
> Detroit, Michigan
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_FQdlfIrcnnwwNdFqKjcIzQ)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_FQdlfIrcnnwwNdFqKjcIzQ)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Ah.The old stew meat vs. lean cuisine!..When we
> get
> spec.that are unfixed..like the ones you have..we run them back thru the
> processor on clean cycle..than return to formalin..and put back on
> processor with the normal run.I realize it delays results a day..But
> hey..They cant read what they submitted anyway..you could try 70% alcohol
> ..let
> the fatty tissue sit in it until processing. You will just have to let the new
>
> residents know..if you do this..to big ect..that's fine..but you wont have
> results for a couple days.Good Luck!
> style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT:
> #000000 2px solid; MARGIN-RIGHT: 0px">
> ----- Original Message -----
> style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color:
> black">
From:
>
Connie
> P.
>
Sent: Monday, April 22, 2002 6:16
> PM
> Subject: Breast specimens
>
> Could we please revisit my current nemesis,
> which
> is surely going to drive me into leaving Histology forever and seeking
> a
> lucrative highly paying successful career?
> I need
> help/advice/suggestions regarding handling breast specimens so
> that
> they are well preserved, easy to cut on the microtome, instead of partially
> raw tissue which requires the cut and scoop method to obtain a slide.
>
> I plead with the residents (1st yrs.) to trim
> them so that they fit in the cassette without touching the sides, and cut
> them
> to 2mm in thickness. Of course, most times they are 4mm or more. They are
> then
> placed into cassettes and held overnight in 10%NBF. They get put on the
> processor the next evening. I tried alcoholic formalin, saw no difference.
> Large breast lymph nodes are just as bad if not worse, raw in the centers.
> The
> small ones are usually fixed O.K. Does anyone have a tried and true method
> for
> handling large breast specimens which works for them with few exceptions? If
>
> so, please share it, I will be forever in your debt.
> Connie L. Probert
> Detroit,
> Michigan
>
> - --Boundary_(ID_FQdlfIrcnnwwNdFqKjcIzQ)--
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 21:31:17 -0500
> From: Angel92764@aol.com
> Subject: Re: sakura tissue TEK coverslipper
>
> What do you mean cornflaking? Bubbles? If it is bubbles, just increase your
> xylene flow. That fixed it for us. We would also have problems with the
> slides jamming in the chute. I found that if I used a cotton tip applicator
> soaked in 100% acetone, I would use this to clean the slide tracking and the
> metal side rails at the top of the slant where the slide would begin it's
> exit downward. This area acquires a large amount of adhesive buildup. You
> will also notice that after a period of time with this particular
> coverslipper, usually about 6 months, if you use the edge of your fingernail
> to pry the edge of the cover-tape, it will just pop right off. This is not a
> good thing. This has been my experience with this instrument. I hope that I
> may have helped you in some way.
>
> Jeanie Wade, H. T. (ASCP)
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 22:00:32 -0500
> From: rueggp
> Subject: Re: Need help for decal samples
>
> amy,
> did your researcher fix the samples before decal?
> if they were not fixed you could have washed out the cells with all that
> washing.
> patsy
>
> Amy Porter wrote:
>
> > We are working on a research project involving mouse bone. Trying to
> > perform Luna's stain for eosinophils. Tissues have been decaled with
> > formic for a predetermined length of time by the researcher, then
> > rinsed in running water for 4 hours and processed by standard
> > methods. The stain seems to be diffusely staining no matter the
> > variations that we try, without much luck seeing the eosinophils. All
> > samples that are formalin fixed without decal are staining
> > beautifully. Any suggestions would be helpful.
> >
> > Amy S.Porter, HT(ASCP)
> > Michigan State University
> > College of Human Medicine
> > portera203@yahoo.com
> >
> >
> > -----------------------------------------------------------------------
> > Do You Yahoo!?
> > Yahoo! Games - play chess, backgammon, pool and more
>
>
>
> ----------------------------------------------------------------------
>
> Date: 22 Apr 2002 22:30:22 -0500
> From: "Ms. Evelyn Kaplan"
> Subject: Re: Charcot-Leyden crystals-birefringence?
>
> Hi Anita,
> Birefringence is exhibited by a substance, often crystalline, whose
> molecular structure is aymetrical so that two rays of light in perpendicular
> planes will travel at different velocities through the substance, producing
> a fast ray and a slow ray. This substance has two refractive indicies and is
> said to show positive birefringence if the plane of vibration of the slow
> ray is parallel to the length of the crystal, or negative if the plane of
> vibration of the slow ray is perpendicular to the length of the fibre.
> Normally birefringent substances appear colourless (white) against a dark
> background under crossed polars, but where the thickness of the crystal of
> fibre is uniform, interference colours may be produced when two rays (fast
> and slow) are reunited in the analyser.
> Dichroism is due to light-absorbing differences along different planes of an
> asymetrical substance. The spatial arrangement of light-absorbing bands
> (resonating electrons) is such that either light of a definite wavelenght is
> selectively absorbed or a change in intensity of white light occurs when
> light passes through the substance in certain planes. The dichroism shown by
> amyloid is a property of the dye molecule, e.g. Congo red or toluidine
> blue, whose linear molecules are bound by hydrogen bonds along the parallel
> folds in the beta-pleated sheet protein, producing different light-absorbing
> characteristics along certain planes of the fibril. Dichroism is studied in
> tissue sections using one polariser only.
> Hope this helps,
>
> Evelyn Kaplan
> Sultan Qaboos University
> Oman
>
>
>
> Here are the messages received yesterday!
>
>
<< Previous Message | Next Message >>