RE: p24

From:"Morken, Tim"

Ann, We use the DAKO HIVp24 antibody on FFPE tissue, digested with
protienase K, 1:10 dilution, 60 min incubation and DAKO LSAB2 detection kit.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043

-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Monday, April 22, 2002 6:24 PM
Subject: p24

Hi there,

Anyone out there is Histoland doing an IHC stain for HIV using antibody p24
from Dako?
I have been trying to work it up on on tissue fixed in Shreks and processed
for a researcher. There are plasma cells present - but the specimen came
from some 3rd world country and has set in this fixative for a few weeks. far, no staining.  Have not been able to determine if it is my
tissue or protocol that is giving me the problem......all suggestions


Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454

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