Ann, We use the DAKO HIVp24 antibody on FFPE tissue, digested with
protienase K, 1:10 dilution, 60 min incubation and DAKO LSAB2 detection kit.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32 
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov 


-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Monday, April 22, 2002 6:24 PM
To: histonet@pathology.swmed.edu
Subject: p24


Hi there,

Anyone out there is Histoland doing an IHC stain for HIV using antibody p24
from Dako?
I have been trying to work it up on on tissue fixed in Shreks and processed
for a researcher. There are plasma cells present - but the specimen came
from some 3rd world country and has set in this fixative for a few weeks.
.......so far, no staining.  Have not been able to determine if it is my
tissue or protocol that is giving me the problem......all suggestions
welcome!

Ann

Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454
amarusk1@fairview.org
612-273-9119