RE: murine CD34

From:"Ward, Elizabeth"

Glen,
There's lots  of info available at abcam.com.Here's a sample:



ab8536 CD34 
Mouse monoclonal to CD34 
WB, IP, PES, FS, IM, IHC, FC 
CD34 QBEND-10 - ab8536
Product type
Primary antibodies
Description
Mouse monoclonal to CD34
 
Clone number
QBEND-10
Target protein
CD34
Immunogen
Human endothelial vesicles.
Epitope
This antibody's target epitope is distinct from those of other anti-CD34
reactive mabs as demonstrated by its lack of sensitivity to neuraminidase
treatment, and fluorescence staining profiles during flow cytometry analysis
of target cell populations. 
Specificity
This antibody recognizes the CD34 antigen, some cross reactions with
vascular associated adventitia and some basement membranes. 
Ultrastructure studies, using an indirect immuno- gold technique on ultra
thin sections of human umbilical artery, have revealed that this antigen is
associated to a degree with the basoluminal surfaces of endothelium and
shows a slight reaction with an intracytoplasmic vesicular compartment.
Predominant staining is on the endothelial cell membrane. 
QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD
expressed on haematopoietic cells and on the established myeloid leukaemic
cell line KG1A. 
Cross Reactivity
This antibody does not cross react with sheep, cow, dog and rat. 
Clonality
monoclonal
Raised in
Mouse
SubClass
IgG1
Purity
tissue culture supernatant
Storage buffer
PBS with 1% BSA and 0.1% sodium azide
Applications
Western blotting, Immunoprecipitation, Immunohistochemisty (Formalin-fixed
paraffin-embedded sections), Immunohistochemistry (Frozen Sections),
Immunomicroscopy, Immunohistochemistry, Flow Cytometry
Uses
1) Suitable for immunohistochemical staining of frozen tissues sections post
fixed with formalin, acetone chloroform-acetone 1:1 at -30C, glutaraldehyde
and methacarn; or formalin fixed paraffin embedded material. 
The antigen is labile to ethanol and methanol, with staining being greatly
reduced or lost 
2) Applications for haematology- bone marrow transplantation: 
Functional assays for specificity against haemapoietic progentior cells were
demonstrated in preliminary clinical studies by its effect on in vitro bone
marrow colony formation. 
Depletion of CD34(+) bone marrow cells, with this antibody substantially
inhibited the growth of colonies. 
Conversely, enrichment of CD34(+) cells, significantly increased the number
of colonies formed by myeloid progenitor cells. QBEND-10 is currently being
evaluated by clinical haematologists for its efficiency in ex vivo
enrichment of haemapoietic stem cells for bone marrow transplantation. 
QBEND-10 was found to selectively label CD34(+) peripheral blood mononuclear
cells during the period of vigorous haemopoietic recovery, in patients
undergoing chemotherapy, by flow cytometry analysis. 
Working dilution for flow cytometry 10 l for 106 cells, a dilution of
approximately 1:5 is advised. 
For immunohistochemistry 1:10 dilution is suggested. 
Applicable working dilutions for immunoprecipitation and Western blotting
should be tested on each individual laboratory.
Positive control
Aceton fixed rat lung endothelial cell (90% RECA positive) stains negative
with this antibody. 
Research areas
References
The anti-CD34 reactivity of QBEND-10 was established in a molecular test for
specificity and the results are published in the proceedings of the 4th.
International Workshop on leucocyte Antigens. 
Fina, L., et al. (1990) Blood 75, 2417-2426. 
Sopper, S., et al (1997). Lymphocyte subsets and expression of
differentiation markers in blood and lymphoid organs of Rhesus monkey.
Cytometry 29: 351-362.

ab8147 CD34 
Mouse monoclonal to CD34 (biotin) 
FC 
ab8148 CD34 
Mouse monoclonal to CD34 (R-PE) 
FC 
ab8158 CD34 
Rat monoclonal to mouse CD34 
WB, IP, ELISA, PES, FS, IF, IHC, FC 
ab8159 CD34 
Rat monoclonal to mouse CD34 (biotin) 
WB, IP, ELISA, PES, FS, IF, IHC, FC 
ab8534 CD34 
Rat monoclonal to CD34 
WB, IP, ELISA, PES, FS, IF, IHC, FC 
ab8535 CD34 
Rat monoclonal to CD34 (biotinylated) 
WB, IP, ELISA, PES, FS, IF, IHC, FC 
ab534 CD34 
Mouse monoclonal to human CD34 
ab535 CD34 
Mouse monoclonal to human CD34 (FITC conjugate) 
FACS 
ab566 CD34 
Mouse monoclonal to human CD34 (PE conjugate) 
FACS 
ab6330 CD34 
Mouse monoclonal to Human CD34 
FACS, IHC 

-----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Monday, April 01, 2002 1:35 PM
To: histonet@pathology.swmed.edu
Subject: RE: murine CD34


All,

One of my pathologists is looking for an antibody called "murine CD34".  All
he knows is that he read about it in a paper and that it is monoclonal
mouse.  Abcam and Immunoquery searches have come up empty.  Any info. would
be much appreciated.

Thank-you,

Glen A. Dawson
Lead IHC Technologist
Milwaukee, WI




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