RE: Mounting Media
|From:||"Leek, Adrian" <ALeek@cytologix.com>|
If pH is very important, I wouldn't use phosphate at pH8.6 - it has hardly
any buffering effect there. Did you mean pH6.8?
From: Plummer, Timothy B. [mailto:Plummer.Timothy@mayo.edu]
Sent: Tuesday, April 24, 2001 2:48 PM
To: 'email@example.com'; histonet
Subject: RE: Mounting Media
Most antibody companies (i.e., Dako, Vector) sell a medium which contains an
antifade medium. Molecular Probes is also a good source. You can also
prepare you own-here is my favorite formulation which also contains DAPI
(Blue fluorescent nuclear counterstain), but it can be left out.
My formulation for DABCO-DAPI Antifade medium is: 25 mg/mL
1,4-diazabicyclo[2.2.2]octane (DABCO) with 0.5mg/mL,
4,6-diamidino-2-phenylindole (DAPI), in 50% glycerol/phosphate buffered
saline, pH 8.6. DABCO is dissolved in 100% glycerol then 2X PBS at pH 8.6
is added in equal volume to the glycerol. The DAPI can be added from a
higher concentration solution stock which can be stored frozen. All
reagents can be purchased from Sigma Chemical. pH is very important.
> -----Original Message-----
> From: firstname.lastname@example.org [SMTP:email@example.com]
> Sent: Tuesday, April 24, 2001 10:34 AM
> To: histonet
> Subject: Mounting Media
> Hi Histonet,
> Does anyone know of a good mounting media to use on immunoflourecent
> with anti-fade in it?
> Christine McBride, M.S.
> Tulane University Medical Center
> Center for Gene Therapy
> 1430 Tulane Ave.
> New Orleans, LA 70112
> Phone:(504) 988-7069
> Fax:(504) 588-5326
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