Fwd: RE: Mounting Media

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> Tim,<br> <x-tab>        </x-tab>Interesting mounting media that I'll give a bash. Do you have a reference or is it personal communication.<br> Ian. <br> <br> <br> <blockquote type=cite class=cite cite>Date: Tue, 24 Apr 2001 13:47:32 -0500<br> From: "Plummer, Timothy B." <Plummer.Timothy@mayo.edu><br> Subject: RE: Mounting Media<br> To: "'cmcbride@tulane.edu'" <cmcbride@tulane.edu>,<br>    histonet <histonet@pathology.swmed.edu><br> <br> Christine,<br> <br> Most antibody companies (i.e., Dako, Vector) sell a medium which contains an<br> antifade medium.  Molecular Probes is also a good source.  You can also<br> prepare you own-here is my favorite formulation which also contains DAPI<br> (Blue fluorescent nuclear counterstain), but it can be left out.<br> <br> My formulation for DABCO-DAPI Antifade medium is:  25 mg/mL<br> 1,4-diazabicyclo[2.2.2]octane (DABCO) with 0.5mg/mL,<br> 4,6-diamidino-2-phenylindole (DAPI), in 50% glycerol/phosphate buffered<br> saline, pH 8.6.  DABCO is dissolved in 100% glycerol then 2X PBS at pH 8.6<br> is added in equal volume to the glycerol.  The DAPI can be added from a<br> higher concentration solution stock which can be stored frozen.  All<br> reagents can be purchased from Sigma Chemical.  pH is very important.<br> <br> Tim Plummer<br> <br> > -----Original Message-----<br> > From:<x-tab> </x-tab>cmcbride@tulane.edu [SMTP:cmcbride@tulane.edu]<br> > Sent:<x-tab> </x-tab>Tuesday, April 24, 2001 10:34 AM<br> > To:<x-tab>   </x-tab>histonet<br> > Subject:<x-tab>      </x-tab>Mounting Media<br> > <br> > Hi Histonet,<br> > <br> > Does anyone know of a good mounting media to use on immunoflourecent<br> > slides <br> > with anti-fade in it?<br> > <br> > Christine<br> > <br> > Christine McBride, M.S.<br> > Tulane University Medical Center<br> > Center for Gene Therapy<br> > SL-99<br> > 1430 Tulane Ave.<br> > New Orleans, LA 70112<br> > cmcbride@tulane.edu<br> > Phone:(504) 988-7069 <br> > Fax:(504) 588-5326</blockquote> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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