"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Tim,<br>
<x-tab> </x-tab>Interesting
mounting media that I'll give a bash. Do you have a reference or is it
personal communication.<br>
Ian. <br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Tue, 24 Apr 2001 13:47:32
-0500<br>
From: "Plummer, Timothy B."
<Plummer.Timothy@mayo.edu><br>
Subject: RE: Mounting Media<br>
To: "'cmcbride@tulane.edu'" <cmcbride@tulane.edu>,<br>
histonet <histonet@pathology.swmed.edu><br>
<br>
Christine,<br>
<br>
Most antibody companies (i.e., Dako, Vector) sell a medium which contains
an<br>
antifade medium. Molecular Probes is also a good source. You
can also<br>
prepare you own-here is my favorite formulation which also contains
DAPI<br>
(Blue fluorescent nuclear counterstain), but it can be left out.<br>
<br>
My formulation for DABCO-DAPI Antifade medium is: 25 mg/mL<br>
1,4-diazabicyclo[2.2.2]octane (DABCO) with 0.5mg/mL,<br>
4,6-diamidino-2-phenylindole (DAPI), in 50% glycerol/phosphate
buffered<br>
saline, pH 8.6. DABCO is dissolved in 100% glycerol then 2X PBS at
pH 8.6<br>
is added in equal volume to the glycerol. The DAPI can be added
from a<br>
higher concentration solution stock which can be stored frozen.
All<br>
reagents can be purchased from Sigma Chemical. pH is very
important.<br>
<br>
Tim Plummer<br>
<br>
> -----Original Message-----<br>
> From:<x-tab> </x-tab>cmcbride@tulane.edu
[SMTP:cmcbride@tulane.edu]<br>
> Sent:<x-tab> </x-tab>Tuesday, April 24, 2001 10:34 AM<br>
> To:<x-tab> </x-tab>histonet<br>
> Subject:<x-tab> </x-tab>Mounting
Media<br>
> <br>
> Hi Histonet,<br>
> <br>
> Does anyone know of a good mounting media to use on
immunoflourecent<br>
> slides <br>
> with anti-fade in it?<br>
> <br>
> Christine<br>
> <br>
> Christine McBride, M.S.<br>
> Tulane University Medical Center<br>
> Center for Gene Therapy<br>
> SL-99<br>
> 1430 Tulane Ave.<br>
> New Orleans, LA 70112<br>
> cmcbride@tulane.edu<br>
> Phone:(504) 988-7069 <br>
> Fax:(504) 588-5326</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>