Re: *#@!sticking base molds HELP!
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 26 Apr 2000, Kelly Pruitt wrote:
> What can I do to keep my base molds from sticking to my blocks? We clean them in xylene and then soap and water. We air dry them. We treat/spray them while they are clean with Histo Prep after they have air dried. I give them a good coating when I use the Histo Prep. The larger molds are usually good for one use. On the biopsy molds, they stick every time. It doesn't matter what I do.
Stand the mould-with-block on the floor of the freezing
compartment of a refrigerator and leave it there for about
a minute. The wax block will then pop out easily. I
suppose the contraction of the metal loosens its adhesion
to the wax. I use Tissue-Tek embedding rings, and find
that the freezer trick is needed for about 50% of all
blocks. It always works, whereas release-compound works
only sometimes.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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