Hi Jim,

We have 2 Ventana immunostainers and were treating the waste with bleach,
letting it sit for 24 hours and then pouring it down the drain.  Recently,
upon looking into a DAB filter waste system and consulting with our
University Safety Department we were told that the DAB was so dilute that
there was no problem letting that go down the drain untreated.  However,
they did have a problem with the Coverslip Oil going down the drain.  So now
we drain off the aqueous portion of the waste into the sink until we're down
to the coverslip layer.  This we drain into a carboy that the safety
department picks up from our lab upon request.

We were concerned about the wrong chemical all along.  Go figure!  

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568

> -----Original Message-----
> From:	Fessler, Jim [SMTP:jifess@pahosp.com]
> Sent:	Wednesday, April 26, 2000 9:08 AM
> To:	'histonet@pathology.swmed.edu'
> Subject:	ventana waste
> 
> I am using both the Ventan ES for immuno and the Nexes Special Stain
> Module
> . I sure would appreciate any feedback as to how users are  disposing the
> waste from each instrument. Thanks, Jim Fessler Pennsylvania Hospital in
> beautiful downtown Philadelphia (the city of brotherly love!)