Freezing microtomes
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From: | "t.hacker@har.mrc.ac.uk" <T.Hacker@har.mrc.ac.uk> |
To: | "'Histonet'" <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | |
Paulo,
You will find that there are freezing microtomes out there gathering
dust but I would go with the suggestions of a portable "cryostat"
(two or three on the market, I am sure the vendors will soon
approach you).
I have used a CO2 freezing microtome many years ago and they
do produce a good quality section (very rapid freezing) and is fine
for a one of section (forget serials) albeit a little thick by todays
standards, but perfectly diagnosable.
If you do get one please be aware of the ferocity of the CO2 jet as
in inexperienced hands I have seen "splats" of tissue on the inner
side of the freezing cover and bits of tissue projected across the
room when the cover is forgotten! (there could also be a safety
issue here,aerosols etc.).
Terry.
Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360
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