To save yourself a lot of work, with a toxic chemical, make up your methyl
green solutions with methyl violet FREE methyl green dye from Sigma, they
have preextracted the MV from the methyl green for you.  Bob Schoonhovens
methyl green is still the superior formulation I have seen, no heating
needed and IT STAINS!  





>Date: Thu, 30 Mar 2000 16:43:51 +0200
>From: Jochen Schuck <jschuck@otogene.de>
>Subject: Re: Antibody pre-absorbtion and Methyl Green
>To: histonet@pathology.swmed.edu
>X-MX-Comment: QUOTED-PRINTABLE message automatically decoded
>
>Wait a minute, wait a minute, my precious liver has other things to do. I
>read that methyl greens are pre-cleared in chloroform.  I did it once and it
>was a glorious mess, but it was clean afterwards.
>Dear Dana, can you tell me what light green is. I mostly now the german
>names and light green doesn´t ring a bell. What does it stain? Where do I
>get it from? How do I use it? I´m still looking for a non-nuclear
>counter-stain...
>Thanks
>Jochen Schuck
>
>> Von: DDittus787@aol.com
>> Antworten an: histonet@pathology.swmed.edu
>> Datum: Wed, 29 Mar 2000 18:07:51 -0500 (EST)
>> An: esabin@srv4.med.ed.ac.uk, histonet@pathology.swmed.edu
>> Betreff: Re: Antibody pre-absorbtion and Methyl Green
>> 
>> Most methly greens need to be pre-cleared in cold acetone and cold benzene,
>> woops there goes your liver, did you try working light green as a
substitute?
>> Dana
>> 
>
>
>
>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303