The how and why's of IHC

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To:"HistoNet Server" <>
Date:Wed, 15 Sep 1999 13:41:08 GMT
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What exactly is the reason(s) some antibodies are great for IHC and some
aren't?  When an antibody works in a Western what is the percentage (rough
estimate) of times it will also work in IHC?  Will IHC Ab's always work in
Westerns?  Why would an antibody work in paraffin sections but not in

Just wondering if anyone has any specific answers for me ...


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