fixative question for chemistry experts

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From:Marcia Bentz <mb7x@virginia.edu>
To:histonet@pathology.swmed.edu
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Date:Fri, 03 Sep 1999 14:25:31 -0400
Content-Type:text/plain; charset="us-ascii"

Hi all,
Just wondering if anyone out there's heard of a fixative called PLP.
It contains:
4% paraformaldehyde dissolved in dH2O plus 2 drops of 10M NaOH
sodium m-periodate (NaIO4)
DL-Lysine
sodium phosphate. I'm not sure of the concentrations, I have a recipe for a
given amount of solution.

My questions are:
Is this approximately the same as dissolving 4% paraformaldehyde in PBS?
What is the difference between this and 10% buffered formalin?

Happy friday and a safe Labor Day holiday to all!

Marcia
(both in terms of preservation of epitopes for immunostaining)




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