fixative question for chemistry experts
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From: | Marcia Bentz <mb7x@virginia.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 03 Sep 1999 14:25:31 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Hi all,
Just wondering if anyone out there's heard of a fixative called PLP.
It contains:
4% paraformaldehyde dissolved in dH2O plus 2 drops of 10M NaOH
sodium m-periodate (NaIO4)
DL-Lysine
sodium phosphate. I'm not sure of the concentrations, I have a recipe for a
given amount of solution.
My questions are:
Is this approximately the same as dissolving 4% paraformaldehyde in PBS?
What is the difference between this and 10% buffered formalin?
Happy friday and a safe Labor Day holiday to all!
Marcia
(both in terms of preservation of epitopes for immunostaining)
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