Many thanks and happy Labor Day to you!!!  Regards, Tom

Karen Larison wrote:
> 
> Tom,
> 
> The general rule of thumb when staining with fluorescent protein
> conjugates is to bracket around 10 micrograms per mL.  When using a good
> fluorescent IgG conjugate, I found that 5 micrograms/mL was a bit dim,
> whereas 20 micrograms/mL often had a bit too much background.  This rule
> of thumb depends somewhat on the fluorophore (some yield a higher
> background, etc), but for TRITC conjugates, 10 micrograms/mL usually
> works great.  Although the molecular weight of your lectin is probably
> is a bit less than IgG, a 2-3 fold difference in molecular weight
> prabably won't make that much of a difference.  I used to use a TRITC
> conjugate of wheat germ agglutinin at 10 micrograms per mL and it
> stained beautifully.
> 
> Karen in Oregon
> 
> 
> Date:          Fri, 03 Sep 1999 09:45:26 -0700
> From:          Tom Kuwahara <tom@adpath.com>
> Subject:       tomato lectins conjugated with TRITC
> To:            Tim Morken <timcdc@hotmail.com>
> Cc:            HistoNet@pathology.swmed.edu
> 
> Hi Tim:  Can you or anybody out there in Histonetland give me a working
> range for lectins conjugated with TRITC for IHC work?  Sigma has no data
> sheet or apparently no info they could give me.  Appreciate any numbers,
> concentrations, etc.  anyone can give.  Thanks, Tom
> 
> >
> 
> --
> *******************************
> Thomas J. Kuwahara
> Senior Immunohistochemist
> Advanced Pathology Systems
> 3801 Sacramento St. suite 621
> San Francisco, CA 94118
> 415 750 6800 x23067 tel
> 415 750 2332 fax
> tom@adpath.com

-- 
******************************* 
Thomas J. Kuwahara
Senior Immunohistochemist
Advanced Pathology Systems 
3801 Sacramento St. suite 621 
San Francisco, CA 94118 
415 750 6800 x23067 tel 
415 750 2332 fax 
tom@adpath.com