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From:"Anne S. van Binsbergen" <anneb@mail.saimr.wits.ac.za>
Date:Sat, 11 Sep 1999 09:14:00 +0000
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Sections are soaked/covered in celloidin PRIOR to staining
Place into celloidin solution from rehydrating absolute alcohol for about
3-5min, drain upright and when 'dry to touch' place in 70% alcohol -3-5min
and thereafter into dist water. Stain with whatever you wish and  from the
dehydration alcohol place into 1:1 ether alcohol until celloidin has
dissolved. Voila!

I kept 25 micron thick 'jumbo' sections of brain on slides during silver
stains most successfully using this technique.
Good luck 
Annie in Africa  
Anne S. van Binsbergen
Laboratory Manager
S.A.I.M.R. Anatomical Pathology
Chris Hani Baragwanath Hospital
PO Box 1038
Johannesburg 2000	
South Africa

Happiness is not the absence of problems
but the ability to deal with them  ;- )

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