Re: Mummified Tissues
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 10 Sep 1999 15:54:05 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear All,
From my notes on museum preparation I found the following methods (some
rather historical!) for mummified tissues:
1: Soak in a saturated sol'n of sodium tribasic phosphate at 50C for at
least 8 hours, perhaps several days.
2: Soak in 50% polyethylene glycol.
3: If badly dried, soak in 1-3% KOH, the process must be watched carefully.
4: Armond Ruffer Technique
Soak in 3:5:2 Ethanol:Water:5% Soda solution, to restore to original texture.
"Science & Secrets of Early Medicine" Jurgen Thorwald, 1962
DuMont Press, Cologne, West Germany, pp35
For museum mounting the tissue is mounted in 10-15% glycerin or
polyethylene glycol - as this softens the tissue.
Regards
Rob W.
R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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