Re: Mummified Tissues

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From:"R.Wadley" <s9803537@pop3.unsw.edu.au>
To:HistoNet@pathology.swmed.edu
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Date:Fri, 10 Sep 1999 15:54:05 +1000
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	Dear All,

	From my notes on museum preparation I found the following methods (some
rather historical!) for mummified tissues:

	1:  Soak in a saturated sol'n of sodium tribasic phosphate at 50C for at
least 8 hours, perhaps several days.

	2:  Soak in 50% polyethylene glycol.

	3:  If badly dried, soak in 1-3% KOH, the process must be watched carefully.

	4:  Armond Ruffer Technique
	Soak in 3:5:2 Ethanol:Water:5% Soda solution, to restore to original texture.
	"Science & Secrets of Early Medicine" Jurgen Thorwald, 1962
	DuMont Press, Cologne, West Germany, pp35

	For museum mounting the tissue is mounted in 10-15% glycerin or
polyethylene glycol - as this softens the tissue.	

	Regards

	Rob W.

R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
E-mail	r.wadley@unsw.edu.au
www	http://www.micro.unsw.edu.au/caf.html



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