Re: manual immunostainers

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From:"Kimberly L Merriam" <>
To:Gayle Callis <>
Date:Mon, 13 Sep 1999 14:18:39 -0400
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Gayle Callis wrote:

> The Shandon coverplate holders are frequently used in this laboratory
> but not the whole Sequenza setup, the holders are adequate, take up little
> space, you can do a hundred slides if you want, each holder costs
> around $160, coverplates can be reused, we do that ad nauseum, unless
> they are scratched and must be carefully washed.
> The amount of buffer is minimal!  10 slides/holder, for a whole
> run used approx 120 mls of buffer, and I have 6 holders and could run
> 60 slides at a time, not bad for hand methods.
> For paraffin, it works nicely, you will need to determine how long
> each antibody needs to be stained with substrate/chromogen development, once
> determined, easy.
> Antigen retrieval and quenching endog peroxidase can be done before mounting
> the slides, for paraffin sections to avoid bubbles.
> You can put holders in refrig, cold room or 37C incubators is desired.
> For efficient, inexpensive staining devices, this was a godsend.
> Gayle Callis

I second the motion on the Shandon system.  We love it, we use an Eppendorf
repeat pipettor to pipet everything, it saves even more time.  Why waste money
on an automatic stainer when you can buy this system for so much less!!!

Kim Merriam

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