Re: ganglia identification in colonoscopy biopsies

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From:Mary Latimer <>
Date:Thu, 2 Sep 1999 14:52:45 +0100 (BST)
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It would not be practical to do esterase histochemistry in minutes. I do
it regularly it is overnight at 37degrees however if you should look into
a flourescent labelled primary that reconises neural tissue and give it a
try this is the quickest way to do ihc.

On Thu, 2 Sep 1999 wrote:

> Acetycholinesterase stain indentifies this.  I have been out of the lab for a 
> while so I'm not sure how long this takes, I only remember it being time 
> consuming.  I am sure some one else will comment.
> Dawn M. Truscott
> In a message dated 09/01/1999 10:04:13 PM Eastern Daylight Time, 
> la.sebree@HOSP.WISC.EDU writes:
> << Hi Histonetters and IPOXers,
>  One of our pathologists has asked us to find a way to identify the presence
>  of ganglia in frozen sections of colon biopsies.  Colonoscopy is performed
>  to identify Hirschsprung's disease.  The idea is to sample sections of colon
>  and perform quick stains to access the presence or absence of ganglia.  I
>  don't know of any immunohistochemistry procedure that can be done within the
>  30-45 minutes time frame.  All of the biopsies would be assessed while the
>  patient is on the O.R. table.
>   >>

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