It would not be practical to do esterase histochemistry in minutes. I do
it regularly it is overnight at 37degrees however if you should look into
a flourescent labelled primary that reconises neural tissue and give it a
try this is the quickest way to do ihc.

On Thu, 2 Sep 1999 DayDawning@aol.com wrote:

> Acetycholinesterase stain indentifies this.  I have been out of the lab for a 
> while so I'm not sure how long this takes, I only remember it being time 
> consuming.  I am sure some one else will comment.
> 
> Dawn M. Truscott
> 
> In a message dated 09/01/1999 10:04:13 PM Eastern Daylight Time, 
> la.sebree@HOSP.WISC.EDU writes:
> 
> << Hi Histonetters and IPOXers,
>  
>  One of our pathologists has asked us to find a way to identify the presence
>  of ganglia in frozen sections of colon biopsies.  Colonoscopy is performed
>  to identify Hirschsprung's disease.  The idea is to sample sections of colon
>  and perform quick stains to access the presence or absence of ganglia.  I
>  don't know of any immunohistochemistry procedure that can be done within the
>  30-45 minutes time frame.  All of the biopsies would be assessed while the
>  patient is on the O.R. table.
>   >>
> 
>