It is important to wash excess picrates out of the tissue after Bouin's 
fixation. Most people use several changes of 50% ethanol, one hour each. 
I suppose the "leaching buffer" is intended to do the same thing?

Geoff

Anthony Gatt wrote:
> Hi. I was given a protocol for Drosophila head fixation that involved Bouin's fixative.  After fixing for 48h, it says to put the samples into a leaching buffer (50mM Tris/150mM NaCl) overnight.  What is the purpose of this buffer?
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff@umdnj.edu
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