RE: [Histonet] Bouin's fixative

From:"Bernice Frederick"

We rinse it in running water.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
[] On Behalf Of Geoff
Sent: Friday, September 28, 2007 3:05 PM
Subject: Re: [Histonet] Bouin's fixative

It is important to wash excess picrates out of the tissue after Bouin's 
fixation. Most people use several changes of 50% ethanol, one hour each. 
I suppose the "leaching buffer" is intended to do the same thing?


Anthony Gatt wrote:
> Hi. I was given a protocol for Drosophila head fixation that involved
Bouin's fixative.  After fixing for 48h, it says to put the samples into a
leaching buffer (50mM Tris/150mM NaCl) overnight.  What is the purpose of
this buffer?
> _______________________________________________
> Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>