RE: [Histonet] Bouin's fixative

From:"Bernice Frederick"



We rinse it in running water.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Friday, September 28, 2007 3:05 PM
To: Anthony.Gatt@jefferson.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bouin's fixative

It is important to wash excess picrates out of the tissue after Bouin's 
fixation. Most people use several changes of 50% ethanol, one hour each. 
I suppose the "leaching buffer" is intended to do the same thing?

Geoff

Anthony Gatt wrote:
> Hi. I was given a protocol for Drosophila head fixation that involved
Bouin's fixative.  After fixing for 48h, it says to put the samples into a
leaching buffer (50mM Tris/150mM NaCl) overnight.  What is the purpose of
this buffer?
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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