have you tried liquid nitrogen?
----- Original Message -----
Sent: Thursday, September 13, 2007 3:14 PM
Subject: [Histonet] Need help with Brain Tumor FS
> Hello All!
> Can anyone recommend a protocol for frozen section technique - to cut
> Brain tumor biopsies.
> The usual ice-crystal artifact sometimes renders the Frozen Sections
> unreadable for intra-operative diagnosis.
> We use a Leica UV1850 cryostat set at -21*
> OCT media
> and cut our FS at 8 microns
> rapid stain H/E
> The pathologist here tends to believe it is our technique that produces
> the artifact.
> fast freezing with spray vs slow freezing??????????
> We have tried a lot of methods to eliminate this problem
> HELP!!!!!!!!!!! I know someone out there has a remedy for the headache
> this has created!!!!
> Thank you all in advance!!!
> I am glad I can pose questions to others who might be dealing with the
> issues I deal with too!
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