What difficulty are you having with the IF?  We airdry in a dessicator,
place in cold acetone for 10 minutes and then airdry again (for a few
minutes), then stain.  Ours turns out perfect every time (all skins)

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, September 07, 2007 10:48 AM
To: 'Mauricio Avigdor'
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Acetone fixation for immunofluorescence protocols

We usually let the frozen slides airdry for 30-120 min and then begin with
IF without fixation. We do IF on human skin (IgG, IgM, IgA, C3c, Fbg). It
seems to work, because there were no complaints. - but our pathologists
haven't got any comparison. ;)

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mauricio
Avigdor
Gesendet: Donnerstag, 06. September 2007 21:42
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Acetone fixation for immunofluorescence protocols

Greetings:

I am having a bit of difficulty with an immunofluorescence protocol (IF). I
am using fresh tissue fixed for 10 minutes in cold acetone. Are there any
fixatives that are well suited to IF protocols? Has anyone attempted to
stain unfixed tissue?
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