RE: [Histonet] CAP Question

From:"Sebree Linda A."


We have a statement in our Laboratory Procedure Manual under "Antibody
Optimization" that reads:  "The selection of avidin biotin (AB) blocking
will be dependent on the specimen source.  Examples of tissues
benefiting from AB block include kidney and liver.  It may be necessary
to have more than one protocol programmed to offer the choice of
blocking or not."

Hope this helps,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
[] On Behalf Of Laurie
Sent: Tuesday, September 05, 2006 4:15 PM
Subject: [Histonet] CAP Question

Can anyone help explain what kind of policy I would need for the
following CAP question:

If the laboratory uses an avidin-biotin complex (ABC) detection system
(or a related system such as streptavidin-biotin or neutravidin-bioton),
is there a policy that addresses nonspecific false positive staining
from endogenous bioton?

Laurie Colbert
Huntington Hospital

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