You are right: it depends on your cleaning cycle.  We do not use formalin on our VIP so we only have xylene and alcohol on the cleaning cycles.  Of course if the last step is something other than xylene it must be stepped back before infiltration. 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Van Binsbergen
Sent: Wednesday, September 06, 2006 9:29 AM
To: Rene J Buesa; Till, Renee; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] tissues in cleaning cycle

I totally disagree- my vip5's cleaning cycle does xylene then absolute and then has a water wash cycle. If I left a block in my wash cycle it would end up in water so I would certainly reprocess it from at least the 95% station!! If I put it straight into the xylene I would put water into my xylene!!!
Just my 5cents worth here in the desert
Annie

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 06 September 2006 17:01
To: Till, Renee; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] tissues in cleaning cycle

Renée:
  A tissue left in the cleaning cycle is one that is already dehydrated and in the "antemedium" (xylene if that is what you use). That is "normal procedure" if you want to "reinfiltrate" the tissue, so your next step would be to put it BRIEFLY in clean xylene and transfer it to be infiltrated with paraffin.
  Very likely tat your tissue can be put in the last xylene station and through the paraffin stations.
  René J.

"Till, Renee"  wrote:
  Hello. Any suggestions on what to do with tissues that were accidentally left in the processor during the cleaning cycle? I would think they need to be re-infiltrated with paraffin at the very least. I don't know if our processor will let us do anything but a full processing run.



Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72002

Office (501)364-2785

Fax (501)364-3161




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