[Histonet] IHC questions
I'm staining rat liver for anti-Brdu next week, AEC with crystal mount. I'm assuming the aquesous Hematoxylin mentioned in my "old" procedure referes to Mayers. Is that correct?
Is 5 minutes sufficient and can scotts be used for bluing or should I use a tap rinse?
I know fixation is routinely 24 hours. To assure complete fixation would 48 hours work ok. I need to do AR anyways. Thanks for everyones answers. Been doing this for over 13 years but only routine. This IHC, IF, and research stuff is a "tad" new.
Thanks again,
Steve
t"T"t
---------------------------------
Yahoo! for Good
Click here to donate to the Hurricane Katrina relief effort.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai
<< Previous Message | Next Message >>