[Histonet] sudan black/bleaching

From:Helen Ilsley


I wonder if any one can help me.
I am using sudan black to suppress my backgound autofluorescence but 
when I want to photograph my section I seem to be getting 
photobleaching especially at high mags. The image under the 
microscope  blurs and when I move away from the area I am left with a 
bleached, blurry ring.
Is this due to the sudan black?

Has anyone ever had this sort of problem before.
I would really appreciate any help.


Helen Ilsley

Chris Barnard Division of Cardiothoracic Surgery

Cape Heart Centre

University of Cape Town Medical School

Cape Town, South Africa

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