[Histonet] sudan black/bleaching
I wonder if any one can help me.
I am using sudan black to suppress my backgound autofluorescence but
when I want to photograph my section I seem to be getting
photobleaching especially at high mags. The image under the
microscope blurs and when I move away from the area I am left with a
bleached, blurry ring.
Is this due to the sudan black?
Has anyone ever had this sort of problem before.
I would really appreciate any help.
Chris Barnard Division of Cardiothoracic Surgery
Cape Heart Centre
University of Cape Town Medical School
Cape Town, South Africa
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