Re: [Histonet] blade sign

From:"renton louise mrs"


bad batches of blades DO occur, although it would seem not all that frequently. It is hard to believe that ALL blocks at your facility  contain hard inclusions (unless you are specifically dealing with bone & calcified tissue).
Other things that might contribute are the way you treat your blade - wiping it with a Kimwipe before cutting for example, or touching it with forceps when picking up sections. Lastly, it may be that there is a contaminant in the wax, dust from nearby renovations might become incorporated in the embedding wax, thus giving rise to scratched & torn sections.

Best regards

-----Original Message-----
From: "jason m" 
Date: Thu, 02 Sep 2004 11:34:40 -0700
Subject: [Histonet] blade sign

anyone have tips for minimizing blade sign? i start at one end of fresh 
blade and work to the other end before throwing out the disposable blade.=20
sometimes however, blade sign appears even with a new blade. is it just that 
some blocks have hard spots that nick the blade?


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Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa what IS the speed of dark?

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