RE: [Histonet] vascular graft_sectioning difficulty

From:"Dr. Raghul"

Hello Aloha,

   Our vascular graft material(DACRON) after fixing in 10% NBF, we 
dehydrate in ascending grades of alcohol(70,80,95,100%),cleared in 3 
changes of chloroform before embedding in paraffin wax (60*C MPT).

   While cutting, the graft area  is powdering in the sections 
obtained.When it comes to the anastomising graft-host vessel part, while 
the vessel portion comes well, the graft as usual gets powdered .

  This is our processing detail and the sectioning difficulty we 
face.Still if the details are insufficient please ask.

regards
raghul

-----Original Message-----
From: "Jack England" 
To: raghul@sctimst.ker.nic.in, Histonet@lists.utsouthwestern.edu
Date: Wed, 15 Sep 2004 08:54:40 -1000
Subject: RE: [Histonet] vascular graft_sectioning difficulty

> Can you provide a little more information about your processing, and a 
> little more detail about how the sections are crumbling (eg, tissue is 
> disaggregating, or the paraffin section is falling apart, etc)? We
> regularly 
> section formalin-fixed, paraffin-embedded vascular grafts (ePTFE) at
> 5-7m, 
> and it's working for us.  With a little more information, I might be
> able to 
> help...
> 
> Aloha,
> Jack England
> Tissue Genesis, Inc.
> http://www.tissuegenesis.com
> 
> >From: "Dr. Raghul" 
> >To: histonet@lists.utsouthwestern.edu
> >Subject: [Histonet] vascular graft_sectioning difficulty
> >Date: Tue, 14 Sep 2004 16:56:23 +0530
> >
> >hello members,
> >
> >We cut 5 m thin sections of paraffin embedded vascular graft(Dacron)
> in
> >a rotary microtome with disposable blades but the senior tech tells
> that
> >the tissue sections are crumbling and forces us to go for thick
> sections
> >around 10m but again not with much success.
> >
> >  We can understand that there is not enough tissue adhereing to the
> >graft and we are literally cutting the graft. But did anyone had such
> >problems sectioning vascular grafts. Any suggestions?
> >
> >
> >regards ,
> >
> >Raghul J
> >scientist
> >Histopathology- implant biology division
> >Srichitra tirunal institute of medical sciences and technology
> >Trivandrum -695 012
> >Kerala, India
> >
> >
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