Re: [Histonet] CD31/PECAM in Rat Brain

From:"Joanne Mauger"

To Danielle,

Pharmingen has a good rat anti ms CD31 catalog# 557355. It works great on ms tissue with pepsin digestion. I use a rabbit anti rat secondary(ms adsorbed)from Vector. Not sure how it will work on rat.
Jo


>>> "Danielle Zalinski"  09/15/04 01:54PM >>>
Hello Histonetters,
Our lab is interested in looking at CD31/PECAM  in rat brain tissue as a 
marker for neovessels.

We don't know anyone in our facility who has used this antibody 
extensively, so we are at a loss for deciding on a manufacturer.  Has 
anyone worked with this antibody and has a (successful) protocol and 
manufacturer that they prefer?
We would prefer a mouse monoclonal for double labeling, but polyclonal 
is ok, too.  Does anyone know if we will need to use any exotic 
fixation/retrieval techniques?  We want to be able to work out a 
protocol first in paraffin and may adapt to frozen sections later.
Thank you for any help you can provide, and if you need more info, 
please let me know,

Danielle Zalinski
Henry Ford Health System
Detroit, MI



CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed.  If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited.  If you received this email in error, please contact the sending party by replying in an email to the sender, delete the email from your computer system and shred any paper copies of the email you printed.

Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. These risks are described in our Privacy Policy at http://henryford.com.  Review that policy carefully before continuing to communicate with us by e-mail. For greater Internet security, our policy describes the Henry Ford MyHealth electronic communication process - you may register at http://henryford.com.  If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us.


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>