Can you provide a little more information about your processing, and a 
little more detail about how the sections are crumbling (eg, tissue is 
disaggregating, or the paraffin section is falling apart, etc)? We regularly 
section formalin-fixed, paraffin-embedded vascular grafts (ePTFE) at 5-7µm, 
and it's working for us.  With a little more information, I might be able to 
help...

Aloha,
Jack England
Tissue Genesis, Inc.
http://www.tissuegenesis.com

>From: "Dr. Raghul" 
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] vascular graft_sectioning difficulty
>Date: Tue, 14 Sep 2004 16:56:23 +0530
>
>hello members,
>
>We cut 5 µm thin sections of paraffin embedded vascular graft(Dacron) in
>a rotary microtome with disposable blades but the senior tech tells that
>the tissue sections are crumbling and forces us to go for thick sections
>around 10µm but again not with much success.
>
>  We can understand that there is not enough tissue adhereing to the
>graft and we are literally cutting the graft. But did anyone had such
>problems sectioning vascular grafts. Any suggestions?
>
>
>regards ,
>
>Raghul J
>scientist
>Histopathology- implant biology division
>Srichitra tirunal institute of medical sciences and technology
>Trivandrum -695 012
>Kerala, India
>
>
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>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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