[Histonet] How obtain a Methyl Green counterstain after a Citrate pretreatment?
|From:||"Rey, Jean-Philippe" |
I try to counterstain some immunostained sections with Methyl green. The one which was pretreated with Citrate buffer at 121degre Celcius, can't been stained with Methyl Green.
Does some one have a clue why? And to be able to obtain some Methyl Green after a slide undergo a Citrate pretreatment?
I will appreciate your help.
Have a nice day
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110
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