[Histonet] Resin embedding-bone matrix

From:Caroline Stott

Hi all,
I don't think this will work but.... I have been asked to resin embed some 
bone that was exposed to treatments of high temps and pressures 
etc.  Consequently I have been left only with the in-organic matrix.  I 
cant decalcify it, or embed in paraffin because there will be nothing 
left,  and I don't think it will cut even if I manage to get it into 
resin.  One of the samples is just powder!!!  (Apparently they are looking 
at a particular protein?)
However the question is, should I make my resin mixture harder than normal 
to accommodate the hard nature of the tissue?  Has anyone tried anything 
similar???  Or do you have another medium I can embed into?
Help and many thanks

Caroline Stott

Histology Service Unit
University of Otago
PO Box 913
Dunedin, New Zealand
Ph  (03) 479 7152
Fax (03) 479 7136 

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