Re: [Histonet] AEC substrate for IHC

From:John Kiernan

The answer is WATER.
You must use an aqueous mounting medium 
to preserve the product of oxidation of
AEC. You must not dehydrate or clear
the preparations.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

Baowei Peng wrote:
> hi, all histoneters,
> We can use ethanol to dehydrate and xylene to clear slides if we used DAB as substrate in IHC. Since AEC is organically soluble, we can not use these reagents.
> I am wondering what reagents we should use and should we dehydrate and clear AEC substrate slides?

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