Re: [Histonet] AEC substrate for IHC

From:Cathy Gorrie

>Dear Baowei,


You will need some sort of water based mountant (available from all 
the IHC companies). Then you coverslip directly from water. The 
slides don't need to be dehydrated through alcohol or cleared in 
xylene.

cheers, Cath
---------------------------------------------------
Catherine Gorrie
School of Medical Sciences
University of New South Wales
Sydney, N.S.W. 2052

Phone: 61-2-9385 2462
Fax  : 61-2-9385 8016
e-mail: c.gorrie@unsw.edu.au

>hi, all histoneters,
>
>We can use ethanol to dehydrate and xylene to clear slides if we 
>used DAB as substrate in IHC. Since AEC is organically soluble, we 
>can not use these reagents.
>I am wondering what reagents we should use and should we dehydrate 
>and clear AEC substrate slides?
>
>Baowei Peng
>Shanghai JiaoTong University,
>Shanghai,
>China,PRC
>
>
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