[Histonet] antigen retrieval buffers

From:"Stylli, Stanley"

Hi Histonetters
I have a question about the longevity of antigen retrieval buffers.
If you make up your own buffers such as the following :
1 mM EDTA (pH 8.0)
10 mM Citrate (pH 6.0)
10 mM tri-sodium citrate (pH 6.0)
100 mM Tris / 1 mM EDTA (pH 9.0)
10 mM Tris (pH 10.0)
How long can these solutions be kept at 4C without affecting the efficiency of the buffer.

Stan Stylli 
Department of Surgery, 
5th Floor Clinical Sciences Building 
Royal Melbourne Hospital 
University of Melbourne 
Parkville, Australia, 3052. 
Tel: 61-3-93427616 


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