[Histonet] RE: IHC with CD25 on FFPE lymph node
Dear Philippe,
The anti-CD25 Cymbus product CBL151 you indicated is in fact clone
Tu69. This clone is unfortunately not working on FFPE sections. In
other words, this particular anti-CD25 clone is reacting with an
epitope on the CD25 molecule that is not surviving the FFPE procedure.
Unfortunately, I am not aware of any anti-CD25 clones working with FFPE
sections.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
>From "Philippe Gannon"
>Date Thu, 04 Sep 2003 17:32:25 +0000
>To HistoNet@pathology.swmed.edu
>Subject [Histonet] IHC with CD25 on FFPE lymph node
>
>hi,
>
>I am having some difficulties with a CD25 staining on formalin-fixed
>paraffin embedded lymph node slides. In fact, I am not seeing any
>staining at all with the antibody CBL 151 from Cymbus Biotechnology
>LTD/Chemicon at concentration up to 1:10 in PBS.
>
>Laboratoire Mes-Masson (Y-4609)
>Institut du cancer de Montréal, Université de Montréal
>2099 Alexandre de Sève
>Montréal, Québec, Canada
>H2L 2W5
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