Re: [Histonet] RE: IHC with CD25 on FFPE lymph node
From: | nick.kirk3@btopenworld.com |
Philippe
Novocastra produce a CD25 that survives paraffin processing. It's clone number 4C9.
It requires Citrate buffer heat mediated antigen retrieval and its code no is NCL-CD25-305
Online catalogue sheet is at http://www.novocastra.co.uk/data/gfr/cd25-305.pdf
The Canadian distributor is Vector labs
Nick Kirk
Histopathology
Hinchingbrooke Hiospital
Huntingdon
England
> from: "C.M. van der Loos"
> date: Mon, 08 Sep 2003 10:07:29
> to: histonet@lists.utsouthwestern.edu
> cc: philippegannon@hotmail.com
> subject: Re: [Histonet] RE: IHC with CD25 on FFPE lymph node
>
> Dear Philippe,
> The anti-CD25 Cymbus product CBL151 you indicated is in fact clone
> Tu69. This clone is unfortunately not working on FFPE sections. In
> other words, this particular anti-CD25 clone is reacting with an
> epitope on the CD25 molecule that is not surviving the FFPE procedure.
> Unfortunately, I am not aware of any anti-CD25 clones working with FFPE=20
> sections.
>
> Chris van der Loos
> Dept. of Cardiovascular Pathology
> Academical Medical Center
> Amsterdam - The Netherlands
>
> >From "Philippe Gannon"
> >Date Thu, 04 Sep 2003 17:32:25 0000
> >To HistoNet@pathology.swmed.edu
> >Subject [Histonet] IHC with CD25 on FFPE lymph node
> >
> >hi,
> >
> >I am having some difficulties with a CD25 staining on formalin-fixed
> >paraffin embedded lymph node slides. In fact, I am not seeing any
> >staining at all with the antibody CBL 151 from Cymbus Biotechnology
> >LTD/Chemicon at concentration up to 1:10 in PBS.
> >
> >Laboratoire Mes-Masson (Y-4609)
> >Institut du cancer de Montréal, Université de Montréal
> >2099 Alexandre de Sève
> >Montréal, Québec, Canada
> >H2L 2W5
>
>
>
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