alcohol-based fixation for PCNA detection

From:Kristel Wautier

Hello everybody,
I have recently read that PCNA is found in two fractions in cells: a fraction associated with the chromosomes and a fraction free in the nucleoplasm. The latter can be "eliminated" by using an alcohol-based fixative, prior to immunohistochemical detection of PCNA.
Does anybody have any suggestions on which fixative would be best. I have been thinking of methacarn. Any suggestions or comments on the quality of morphology when using alcohol-based fixatives?
Kristel Wautier
Ghent University
Department of Biology
K.-L. Ledeganckstraat 35
9000 Ghent
Tel.: +32 9 264 52 31
Fax: +32 9 264 53 44

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